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利用色谱-质谱联用技术对我国主要的3个品种柚子果实的不同部位（外表皮、内表皮、内果皮、果肉、种子）的化学组分进行结构鉴定及种类和含量的差异性分析，结果表明，沙田柚、琯溪蜜柚、玉环柚果实5个部位共鉴定出68种不同化学组分物质，包括醇类19种、酯类15种、烯烃类9种、有机酸类8种、酮类7种、烷烃类4种、其他物质6种。其中3个品种柚子外表皮共有的化学组分有7种，含量最高的是柠檬烯（44.03％）；内表皮共有的化学组分有6种，含量最高的是油酸酰胺（44.62％）；内果皮共有的化学组分有10种，含量最高的是谷甾醇（30.75％）；果肉共有的化学组分有11种，含量最高的是软脂酸（20.02％）；种子中共有的化学组分有15种，含量最高的是亚油酸（45.83％）。化学组分差异性分析结果表明，沙田柚、琯溪蜜柚、玉环柚3种柚子的外表皮独有的化学组分分别有3种、6种、4种，内表皮分别有3种、4种、8种，内果皮分别有1种、4种、6种，果肉分别有4种、4种、2种，沙田柚、玉环柚种子独有的化学组分均为1种，并对化学组分进行聚类分析和相似度分析。研究结果说明不同品种柚子同一部位的化学组分种类和含量均存在相似性和差异性，特有物质较多，色谱指纹图谱特异性显著，这可为柚类品种鉴定、品质质量控制、原产地溯源等提出一种新的色谱指纹图谱思路。

The chemical constituents in different tissues( primary cuticula,epidermis,peel, pulp,seed) of the three kinds of pomelo( Shatian pomelo,Guanxi honey pomelo,Yuhuan pomelo)in China were qualitatively and quantitatively analyzed by gas chromatography-mass spectrometry, liquid chromatography-electrospray ion trap-mass spectrometry and ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry. The results showed that 68 different chemical constituents were identified in the five tissues of the three kinds of pomelo(19 alcohols,15 esters,9 olefins,8 organic acids,7 ketones,4 alkanes and 6 other substances). Among which 7 common constituents were found in primary cuticula,and the highest content was 44. 03%( limonene). In epidermis,the highest content in 6 common constituents was 44. 62%( oleic acid amide ). In 10 common constituents of peel,the highest content was 30. 75%( sitosterol). The highest content was 20. 02%( palmitic acid)of 11 com-mon constituents in pulp. In seed,the highest content of 15 common constituents was 45. 83%(α-linoleic acid ). We also applied the cluster analysis and similarity analysis to the chemical constituents of different parts of the three kinds of pomelo. It is apparent that there are differ-ences and similarities in species and contents of the constituents in the same parts of different varieties of pomelo. The chromatographic fingerprint specificity is obvious,which is helpful to put forward a new idea of chromatographic fingerprint in classification,quality control and geo-graphical tracing of pomelo.