肝微粒体中的药物代谢酶是参与药物代谢的主要酶类，不管是合成药物，还是组成复杂的中药，主要经过肝脏中药物代谢酶的代谢转化成易于排泄的化合物。由于药物代谢酶的表达水平具有较大的个体差异性，因此，药物代谢酶的准确定量对药物的药理、药物相互作用和临床应用具有重要意义。本文综述了近十年来药物代谢酶定量方法的研究进展。
Cytochrome P450( CYP)enzymes and uridine 5-diphospho-glucuronosyltransferase ( UGT)enzymes are critical enzymes for drug metabolism. Both chemical drugs and traditional Chinese medicines are converted to more readily excreted compounds by drug metabolizing enzymes in human livers. Because of the disparate expression of CYP and UGT enzymes among different individuals,accurate quantification of these enzymes is essential for drug pharmacolo-gy,drug-drug interactions and drug clinical applications. The research progress in quantitative methods based on liquid chromatography-mass spectrometry for drug metabolizing enzymes in human liver microsomes in the recent decade is reviewed.
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