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建立了养殖水体及沉积物中11种磺胺化合物的高效液相色谱?柱后衍生分析方法。养殖水体过滤后采用HLB固相萃取柱进行净化、富集；沉积物采用甲醇／EDTA?Mcllvaine缓冲液（1∶1， v／v）提取，HLB固相萃取柱净化富集。经高效液相色谱分离，用荧光胺衍生试剂进行柱后衍生，荧光检测器检测。对柱后衍生系统参数进行了优化，确定了荧光胺溶液的浓度、流速和反应温度分别为0?2 g／L、0?15 mL／min和50℃，磺胺化合物在0?01～1?0 mg／L范围内线性显著，其相关系数r2值大于0?99995。11种磺胺类药物在养殖水体和沉积物中的加标回收率分别为79?3％～100?7％和74?6％～95?3％，相对标准偏差为2?2％～11?0％和2?6％～10?3％，检出限（ LOD， S／N＝3）为0?9～5?5 ng／L和0?3～1?3μg／kg，定量限（ LOQ， S／N＝10）为3?0～18?1 ng／L和1?0～4?4μg／kg。该法可应用于养殖环境中磺胺类药物的定性定量检测，具有较好的实用性。

An analytical method was developed for the determination of 11 sulfonamide com?pounds in aquaculture water and sediments by high performance liquid chromatography ( HPLC) coupled with post?column derivatization. The filtered water sample was purified and concentrated with HLB cartridge, while the sediment sample was extracted with a mixture of methanol and EDTA?Mcllvaine buffer (1∶1, v/v), and then purified and enriched through HLB solid?phase extraction. The sulfonamides were separated on a C18 column by HPLC and on?line derivatized with a fluorescamine and detected with a fluorescence detector. The parameters of post?column derivatization system were optimized, and the fluorescamine solution concentra?tion, velocity of reagent solution and reaction temperature were 0?2 g/L, 0?15 mL/min and 50℃, respectively. The calibration curves of the method showed good linearity in the range of 0?01-1?0 mg/L, with the correlation coefficients ( r2 ) all above 0?999 95. The recoveries were 79?3%-100?7% and 74?6%-95?3% with RSD values of 2?2%-11?0% and 2?6%-10?3% for the 11 sulfonamides in aquaculture water and sediments, respectively. The respective limits of detec?tion ( LODs, S/N=3) were 0?9-5?5 ng/L and 0?3-1?3 μg/kg and the limits of quantification ( LOQs, S/N=10) were 3?0-18?1 ng/L and 1?0-4?4 μg/kg. The method can be applied to the determination of sulfonamides in the aquaculture environment, and it has a good practicability.