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以纤维素-三（3，5-二甲苯基氨基甲酸酯）为手性固定相（Chiralcel OD-H）在高效液相色谱上拆分了氨鲁米特对映体。通过测定氨鲁米特在正己烷/乙醇和正己烷/异丙醇中的溶解度，优选了对样品溶解度大的流动相体系，并考察了流动相添加剂乙醇胺对拆分效果的影响。在此基础上进一步研究了流动相中乙醇含量、柱温和进样量对分离因子、分离度、不对称因子和理论板数的影响，从而确定了最佳的拆分条件：固定相为 Chiralcel OD-H，流动相为正己烷/乙醇/乙醇胺（体积比为30：70：0.1），柱温25℃。本文所得结果可为工业放大提供基础数据。

Aminoglutethimide(AG)has been used clinically as a drug in the treatment of hor-mone-dependent metastatic breast cancer. It was reported that S-( -)-AG enantiomer had small activity and sometimes might cause side effects. Therefore,it was of great significance to obtain the high-purity R-( +)-AG by enantioseparation. In this work,aminoglutethimide enanti-omers were separated by high performance liquid chromatography( HPLC)using an analytical column which was packed with cellulose tris(3,5-dimethylphenylcarbamate)stationary phase (ChiralcellOD-H). The solubilities of racemic AG in two different solvent compositions,n-hex-ane / ethanol and n-hexane / isopropanol,were measured,separately. The effects of alcohol con-tent and monoethanolamine additive on the separation performance of racemic AG by HPLC were investigated. According to the experiments,n-hexane-ethanol( 30 : 70,v / v)with 0. 1%monoethanolamine additive was selected as the mobile phase. The separation factor,resolu-tion,asymmetry factor,number of theoretical plates and maximum column capacity were measured and analyzed for the chromatographic separation of racemic AG at a flow-rate of 0. 6 mL / min and column temperature of 25-40 ℃ ,with ChiralcellOD-H as stationary phase and n-hexane-ethanol(30 : 70,v / v)with 0. 1% monoethanolamine as mobile phase. This work pro-vides the basic information of chromatographic separation for the batch and continuous produc-tion of aminoglutethimide enantiomers.