建立了同时测定中成药和保健品中9种抗抑郁药物的超高效液相色谱-串联质谱（ UPLC-MS / MS）分析方法。以甲醇为提取溶剂，超声提取，离心后取上清液，使用 Waters ACQUITY UPLC BEH C 18色谱柱（100 mm×2.1 mm，1.7μm）进行分离，以乙腈和0.1％（v / v）甲酸水为流动相对目标物进行梯度洗脱。在电喷雾正离子模式下，采用多反应监测（MRM）模式进行定性和定量分析。结果表明，9种抗抑郁药物在0.04~20μg / L 范围内线性关系良好，线性相关系数（R2）均大于0.998；目标物的检出限为0.0068~0.034μg / L；在0.5、1、5μg / L 3个添加水平下的平均回收率为77.7％~100.8％，相对标准偏差为0.9％~9.1％。本方法准确、简便、快速，可用于中成药和保健品中抗抑郁药物的检测。
A sensitive method was developed for the simultaneous determination of nine antide-pressants in traditional Chinese medicines and health food by ultra performance liquid chroma-tography-tandem mass spectrometry( UPLC-MS / MS)based on the sonication-assisted extrac-tion. The separation was performed on a Waters ACQUITY UPLC BEH C 18 column(100 mm×2. 1 mm,1. 7 μm)by using 0. 1%(v / v)formic acid aqueous solution and acetonitrile as the mobile phases with gradient elution at a flow rate of 0. 2 mL / min. The electrospray ionization( ESI) source in positive ion mode was used for the analysis of the nine antidepressants in the multiple reaction monitoring( MRM ) mode. The standard curves for the nine antidepressants were obtained with good correlation coefficients( R 2 ﹥0. 998)in the concentration range of 0. 04-20μg / L. The limits of detection for the nine antidepressants were in the range of 0. 006 8- 0. 034μg / L. The average recoveries at three spiked levels( 0. 5,1,5 μg / L)were 77. 7% - 100. 8%with the relative standard deviation of 0. 9% -9. 1% . The method is accurate,simple,rapid and feasible for the simultaneous determination of the antidepressants in traditional Chinese medi-cines and health food.
参考文献
| [1] | Zhang H,Wang D X. China Journal of Modern Medicine(章虹,汪道鑫. 中国现代医学杂志),2010,20(9):1342,2010. |
| [2] | Gao S. China Journal of Modern Medicine(高山. 中国现代医学杂志),2012,22(33):83,2012. |
| [3] | Hao J,Liu J. Chinese Pharmaceutical Journal(郝杰,柳洁.中国药学杂志),2013,48(11):934,2013. |
| [4] | Xiao L H,Liu J J,Guan X Y,et al. China Modern Medicine (肖丽和,刘吉金,关潇滢,等. 中国当代医药),2009,16 (14):15,2009. |
| [5] | Fernández P,González C,Pena M T,et al. Anal Chim Acta,2013,767:88,2013. |
| [6] | Wu H Q,Jin Y C,Cai M Z,et al. Chinese Journal of Analyti-cal Chemistry(吴惠勤,金永春,蔡明招,等. 分析化学), 2007,35(4):500,2007. |
| [7] | Liu J J,Xiao L H,Guan X Y,et al. Chinese Journal of Phar-maceutical Analysis(刘吉金,肖丽和,关潇滢. 等. 药物分析杂志),2011,31(2):376,2011. |
| [8] | Cartiser N,Bévalot F,Le Meur C,et al. J Chromatogr B, 2011,879(27):2909,2011. |
| [9] | Zhu L,Ruan L P,Liu H L,et al. Chinese Journal of Chro-matography(朱琳,阮丽萍,刘华良. 色谱),2013,31(7):709,2013. |
| [10] | Yan A H,Li X L,Xi C X,et al. Chinese Journal of Analyti-cal Chemistry(严爱花,李贤良,郗存显,等. 分析化学), 2013,41(4):509,2013. |
| [11] | Wang J W,Huang X L,Cao J,et al. Chinese Journal of Chromatography(王静文,黄湘鹭,曹进,等. 色谱),2014, 32(2):151,2014. |
| [12] | Yan L J,Zhang F,Wu M,et al. Journal of Instrumental Analysis(严丽娟,张峰,吴敏,等. 分析测试学报),2011, 30(11):1301,2011. |
| [13] | Li B,Liu W,Fan S,et al. Chinese Journal of Chromatogra-phy(李兵,刘伟,范赛,等. 色谱),2012,30(6):584,2012. |
| [14] | Ming D S,Heathcotea J. J Chromatogr B,2011,879(5/6):421,2011. |
| [15] | Choong E,Rudaz S,Kottelat A,et al. J Chromatogr B, 2011,879(19):1544,2011. |
| [16] | Chambers E E,Woodcock M J,Wheaton J P. J Pharma-ceut Biomed,2014,88:660,2014. |
| [17] | Amundsen I,iestad M L,Ekeberg D,et al. J Chroma-togr B,2013,927:112,2013. |
| [18] | European Commission Decision 2002/657/EC,2002. |
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