建立了被动吸烟儿童尿液中可丁宁的气相色谱-三重四极杆串联质谱（ GC-MS / MS）稳定同位素稀释测定方法。尿液经过三氯甲烷提取、净化，采用气相色谱-三重四极杆串联质谱多反应监测（ MRM）模式测定，以可丁宁-d3稳定同位素为内标，定量测定和确证被动吸烟儿童尿液中的可丁宁；在0.1~10μg / L 可丁宁质量浓度范围内方法的线性关系良好，相关系数 r﹥0.998；空白尿液中添加可丁宁0.1、1.0和10μg / L，回收率为79.2％~112.8％，相对标准偏差在2.1％~5.8％之间；方法定量限达到0.1μg / L。该方法准确、灵敏、快速，适用于家庭被动吸烟儿童尿液中可丁宁的测定。
An analytical method for the determination of urinary cotinine of children exposed to passive smoking was established based on stable isotope dilution by gas chromatography-triple quadrupole mass spectrometry( GC-MS / MS). The samples were extracted and purified with chloroform. The extracts were determined by GC-MS / MS in multiple reaction monitoring (MRM)mode. The cotinine-d 3 as an isotope internal standard was applied to quantify and con-firm the urinary cotinine of children exposed to passive smoking. The method had a good linear-ity from 0. 1 μg / L to 10 μg / L with the correlation coefficient(r)﹥0. 998. The recoveries of the cotinine in blank urine were from 79. 2% to 112. 8% at spiked levels of 0. 1,1. 0 and 10 μg / L, with relative standard deviations(RSDs)from 2. 1% to 5. 8% . The limit of quantification(LOQ) of the method was 0. 1 μg / L. The developed method is accurate,sensitive,rapid and can be applied to detect urinary cotinine of children exposed to passive smoking at home.
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