建立了畜禽产品中维吉尼亚霉素M1和S1药物残留检测的液相色谱-串联质谱分析方法.样品以甲醇-乙腈溶液(1:1,V/v)提取,上清液经0.01 mol/L磷酸二氢铵溶液稀释后,Oasis HLB固相萃取小柱净化,Luna C18色谱柱分离,以乙腈和含0.1％(体积分数)甲酸的5 mmol/L乙酸铵水溶液作为流动相进行梯度洗脱,电喷雾正离子模式电离(ESI+).多反应监测(MRM)模式检测,外标法定量.该方法对两物质线性范围均为0.15～10.O μg/L,相关系数r2均大于0.999；定量下限均为0.25 μg/kg.在不同基质中,0.25、0.50、2.5 μg/kg 3个添加水平的平均回收率范围为71.2％～98.4％,精密度范围为3.6％～15.4％.该方法具有快速简便、灵敏度高、准确性强等特点,适用于畜禽产品中维吉尼亚霉素的检测.
参考文献
| [1] | Council Regulation (EC) No.2821/98,Official Journal of the European Communities,1998,L351:4 |
| [2] | European Commission Decision 2005/34/EC,Official Journal of the European Union,2005,L16:61 |
| [3] | Situ C,Elliott C T.Anal Chim Acta,2005,529(1/2):89 |
| [4] | H-ajee C A J,van Rhijn H A,Lasaroms J J P,et al.Analyst,2001,126:1332 |
| [5] | GB/T 22261-2008 |
| [6] | Geng Z M,Chen M,Xu D G,et al.Chinese Journal of Veterinary Drug(耿志明,陈明,许大光,等.中国兽药杂志),2005,39(2):10 |
| [7] | Yamada R,Kozono M,Ohmori T,et al.Biosci Biotechnol Biochem,2006,70(1):54 |
| [8] | BoisonJ,Lee S,Gedir R.J AOAC Int,2009,92(1):329 |
| [9] | GB/T 20765-2006 |
| [10] | GB/T 22991-2008 |
| [11] | Schneider M J,Mastovska K,Lehotay S J,et al.Anal Chim Acta,2009,637:290 |
| [12] | Lin W X,Sun X Q,Tian M,et al.Journal of Instrumental Analysis(林维宣,孙兴权,田苗,等分析测试学报),2009,28(2):212 |
| [13] | Chen X X,Yue Z F,Ye W X,et al.Journal of Instrumental Analysis(陈小霞,岳振峰,叶卫翔,等.分析测试学报),2009,28(11):1328 |
| [14] | Chen X X,Yue Z F,Ye W X,et al.Food and Fermentation Industries(陈小霞,岳振峰,叶卫翔,等.食品与发酵工业),2010,36(4):175 |
| [15] | Tang Y Y,Lu H F,Lin H Y,et al.J ChromatogrB,2012,881/882:12 |
| [16] | Huang Y H.Chinese Journal of Chromatography (黄永辉.色谱),2011,29(10):962 |
| [17] | Matuszewski B K,Constanzer M L,Chavez-Eng C M.Anal Chem,2003,75:3019 |
| [18] | US Department of Health and Human Services,Food and Drug Administration.Fed.Regist.2001,66(100):28526 |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%