建立了高效、经济、大规模获得鸡卵黄免疫球蛋白(IgY)的生产方法.在对传统的水稀释法改良的基础上,结合聚乙二醇沉淀与离子交换色谱进行IgY的分离纯化.结果显示,用8倍无菌水稀释蛋黄液,用0.1 mol/L HC1调节pH为5.2,在4℃下静置8h,于5 000×g力离心可得上清粗IgY液,经测定回收率可达93.47％.然后用6％聚乙二醇沉淀后经DEAE-Toyopearl 650 M离子交换纯化,最佳的纯化条件:0.05 mol/L磷酸盐缓冲液(PBS,pH 7)平衡上样,0.075 mol/L PBS(pH 7)洗脱.十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)分析结果显示所得的IgY的纯度为95.02％,活性保持率高达73.77％.本研究弥补了传统分离方法不能同时达到高纯度和高回收率的缺点,且可用于大规模生产.
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