应用高速逆流色谱法分离制备了生附子中的3个C<,19>型二萜生物碱类化合物.以正己烷-乙酸乙酯-甲醇-水(3:5:4:5,v/v/v/v)为两相溶剂系统,上相为固定相,下相为流动相,在主机转速850 r/min、流动相流速2.0mL/min、检测波长235 nm条件下进行分离制备;一次性从90 mg附子总碱粗提物中分离制备得到15.3 mg北草乌碱,35.1 mg中乌头碱和22.7 mg次乌头碱,经高效液相色谱分析,测得它们的纯度分别为97.9%、96.2%和99.2%.并应用波谱(电喷雾离子质谱、核磁共振氢谱和核磁共振<'13>C谱)解析法确定了它们的结构.利用该方法可以对生附子中的二萜类生物碱成分进行快速的分离和纯化.
参考文献
| [1] | Pharmacopoeia Commission of People' s Republic of China.Pharmacopoeia of People' s Republic of China.Part 1.Beijing:Chemical Industry Press(国家药典委员会.中华人民共和国药典.一部.北京:化学工业出版社),2005:132 |
| [2] | Murayama M,Mori T,Bando H,et al.J Ethnopharmacol,1991,35(2):159 |
| [3] | Friese J,Gleitz J,Gutser U T,et al.Eur J Pharmacol,1997,337(2/3):165 |
| [4] | Kimura M,Muroi M,Kimura I,et al.Japan J Pharmacol,1988,48:290 |
| [5] | Dong Y L,Chen W Z,Ding G S.Acta Pharmacologica Sinica(董月丽,陈维洲,丁光生.中国药理学报),1981,2(3):173 |
| [6] | Chan T Y,Tomlinson B,Tse L K,et al.Vet Hum Toxicol,1994,36(5):452 |
| [7] | Fu M,Wu M,Wang J F,et al.Biochem Biophy Res Commun,2007,354(4):929 |
| [8] | Yang F Q,Ito Y.J Chromatogr A,2002,943:219 |
| [9] | Jiang K,Yang C H,Liu J H,et al.Acta Pharmaceutica Sinica(蒋凯,杨春华,刘静涵,等.药学学报),2006,41(2):128 |
| [10] | Tang Q F,Ye W C,Liu J H,et al.J ChromatogrA,2007,1144:203 |
| [11] | Tang Q F,Liu J H,Xue J,et al.J Chromatogr B,2008,872:181 |
| [12] | Tang Q F,Yang C H,Ye W C,et al.Phytochem Anal,2008,19:155 |
| [13] | Wu P L,Liu W,Zhuo C,et al.World Science and Technology:Modernization of Traditional Chinese Medicine and Materia Medica(吴平丽,刘雯,卓超,等.世界科学技术:中药现代化),2009,11(2):260 |
| [14] | Hong B,Si Y S,Zhao H F,et al.Journal of Jilin Agriculrural University(洪波,司云珊,赵宏峰,等.吉林农业大学学报),2004,26(1):57 |
| [15] | Wang Y G,Zhu Y L,Zhu R H.Acta Pharmaceutica Sinica (王永高,朱元龙,朱任宏.药学学报),1980,15(9):526 |
| [16] | Pelletier S W,Mody N V,Joshi B S,et al.Alkaloids Chem Biol Persp,1984,2:206 |
| [17] | Shim S H,Kim J S,Kang S S,et al.Arch Pharm Res,2003,26 (9):709 |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%