建立了一种可用于中药寡糖分离的毛细管区带电泳(CZE)分析方法.分离条件:未涂层熔融石英毛细管柱(48 cm x50 μm,有效长度38 cm),紫外检测波长245 nm,采用1-苯基-3-甲基-5-吡唑啉酮(PMP)为寡糖衍生试剂,50 mmol/L磷酸盐溶液(pH 2.5)为运行缓冲液,电压15 kV,重力进样10 cm×2 s.针对中药寡糖实际样品的复杂性,通过添加多种常见单糖进行方法的实用性验证,并且将方法用于板蓝根多糖的控制降解产物的分离.结果表明,板蓝根寡糖组分可按相对分子质量从小到大的顺序分离,分离效果令人满意.该分离方法操作简单、高效,可用于中药寡糖实际样品分析.另外,还对单糖、寡糖PMP衍生物的电泳迁移行为进行了初步的理论探讨.
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