以十二烷基甲基丙烯酸酯(LMA)为功能单体,乙叉二甲基丙烯酸酯(EDMA)为交联剂,正丙醇、1,4-丁二醇和水为三元致孔剂,以及2-丙烯酰胺-2-甲基丙磺酸(AMPS)为电渗流产生剂,制备了聚十二烷基甲基丙烯酸酯整体柱.系统考察了AMPS含量和单体-致孔剂比例对柱性能的影响.结果表明,单体溶液和致孔剂的最佳聚合溶液质量比为35:65,其中单体溶液组成为59.5%(质量分数,下同)LMA、40%EDMA和0.5%AMPS,致孔剂溶液组成为60%正丙醇、30%1,4-丁二醇和10%水.在优化的流动相条件下应用制备的整体柱采用毛细管电色谱法成功地分离了肌红蛋白酶解产物.
The monolithic polylaurylmethacrylate column was prepared in a single step using the monomer solution containing lauryl methacrylate (LMA),ethylene dimethacrylate (EDMA),and 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS),with the ternary porogenic solvent consisting of 1-propanol,1,4-butanediol and H_2O.The effects of AMPS content and the ratio of monomer solution to porogenic solvent were investigated.The optimal mass ratio of monomer solution to porogenic solvent was 35:65,the monomer solution was composed of 59.5%(w/w) LMA,40%(w/w) EDMA and 0.5%(w/w) AMPS,and the porogenic solution was composed of 60%(w/w) 1-propanol,30%(w/w) 1,4-butanediol and 10%(w/w) H_2O.The prepared monolithic column was successfully applied in the capillary electrochromatographic (CEC) separation of myoglobin digests under the optimized mobile phase.
参考文献
| [1] | Yin J F,Wei X Y,Yang G L.Chinese Journal of Chromatography (尹俊发,魏晓奕,杨更亮.色谱),2007,25(2):142 |
| [2] | Zhang S H,Huang X,Zhang J,et al.J Chromatogr A,2000,887:465 |
| [3] | Lee D,Svec F,Fréchet J.J Chromatog A,2004,1051:53 |
| [4] | Hemstr?m P,Nordborg A,Irgum K,et al.J Sep Sci,2006,29:25 |
| [5] | Ding M Y,Zheng R,Peng H.Chinese Journal of Analytical Chemistry (丁明玉,郑睿,彭虹.分析化学),2009,37(3):395 |
| [6] | Wu R A,Zou H F,Ye M L,et al.Anal Chem,2001,73:4918 |
| [7] | Jiang X G,Dong J,Wang F J,et al.Electrophoresis,2008,29:1612 |
| [8] | Xie J X,Bi K S,Qian X H,et al.Chinese Journal of Chromatography (谢晶鑫,毕开顺,钱小红,等.色谱),2009,27(2):186 |
| [9] | Ping G C,Zhang W B,Zhang L H,et al.Chromatographia,2003,57:629 |
| [10] | Lin J,Wu X P,Lin X C,et al.J Chromatogr A,2007,1169:220 |
| [11] | Adu J K,Lau S S,Watson D G,et al.Electrophoresis,2005,26:3445 |
| [12] | Gu B H,Chen Z Y,Thulin C D.Anal Chem,2006,78:3509 |
| [13] | Ping G C,Kopplin P S,Hertkorn N,et al.Electrophoresis,2003,24:958 |
| [14] | Ping G C.[PhD Dissertation].Dalian:Dalian Institute of Chemical Physics,Chinese Academy of Sciences (平贵臣.[博士学位论文].大连:中国科学院大连化学物理研究所),2003:32 |
- 下载量()
- 访问量()
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%