采用固定化脂肪酶Candida sp.99-125在无溶剂体系中催化合成油酸低碳醇酯,考察了加酶量、温度、酸醇摩尔比和醇结构对油酸酯化率的影响.结果表明,加酶量为底物质量的3％,最适温度为20℃,酸与醇摩尔比为1∶1,甲醇对该酶有一定的毒性,由于空间位阻效应,该酶对伯醇具有高选择性,对仲醇、叔醇的选择性低,且对长链脂肪酸催化活性高,对带支链的多元酸、多元醇活性低.该酶重复使用5次,酶活性基本没有降低.与传统的化学法相比,用该酶催化合成酯类化合物的色泽更浅.
参考文献
| [1] | He X L,Chen B Q,Tan T W.Enzymatic Synthesis of 2-Ethylhexyl Esters of Fatty Acids by Immobilized Lipase from Candida sp.99-125[J].J Mol Catal B:Enz,2002,18(4/6):333-339. |
| [2] | Kobayashi T.Lipase-Catalyzed Syntheses of Sugar Esters in Non-Aqueous Media[J].Biotechnol Lett,2011,33 (10):1911-1919. |
| [3] | Samey D B,Barnard M J,MacManus D A,et al.Application of Lipase to the Regioselective Synthesis of Sucrose Fatty Acid Monoesters[J].J Am Oil Chem Soc,1996,73 (11):1481-1487. |
| [4] | Valivety R H,Johnston G A,Suckling C J,et al.Solvent Effects on Biocatalysis in Organic Systems:Equilibrium Position and Rates of Lipase Catalyzed Reactions[J].Biotechnol Bioeng,1991,38(10):1137-1143. |
| [5] | Ferrer M,Cruces M A,Plou F J,etal.A Simple Procedure for the Regioselective Synthesis of Fatty Acid Esters of Maltose,Leucrose,Maltotriose and n-Dodecyl Maltosides[J].Tetrahedron,2000,56 (24):4053-4061. |
| [6] | Tan T W,Chen B Q,Ye H.Enzymatic Synthesis of 2-Ethylhexyl Palmitate by Lipase Immobilized on Fabric Membranes in the Batch Reactor[J].Biochem Eng J,2006,29 (1/2):41-45. |
| [7] | Neta N A S,Santos J C,Sancho S O,et al.Enzymatic Synthesis of Sugar Esters and Their Potential as Surface-active Stabilizers of Coconut Milk Emulsions[J].Food Hydrocolloids,2012,27(2):324-331. |
| [8] | NI Wanxing,ZHANG Xiaoming.Lipase-Catalyzed Synthesis of Bio-Diesel in Solvent Free System[J].Food and Machinery,2007,23(1):25-28 (in Chinese).倪婉星,张晓鸣.非水相脂肪酶催化合成生物柴油[J].食品与机械,2007,23(1):25-28. |
| [9] | JING Liang,XU Yan,CAO Guangqun.Biosynthesis of Oleyl Oleate by Whole-Cell Lipase from Rhizopus Chinensis[J].Chinese J Catal,2006,27 (7):611-614(in Chinese).金亮,徐岩,曹光群.华根霉全细胞脂肪酶催化合成油酸油醇酯[J].催化学报,2006,27(7):611-614. |
| [10] | DENG Li,TAN Tianwei,WANG Fang.Studies of Enzymatic Synthesis of Biodiesel[J].Chinese J Biotechnol,2003,19(1):97-101 (in Chinese).邓力,谭天伟,王芳.脂肪酶催化合成生物柴油的研究[J].生物工程学报,2003,19(1):97-101. |
| [11] | Nie K L,Xie F,Wang F.Lipase Catalyzed Methanolysis to Produce Biodiesel:Optimization of the Biodiesel Production[J].J Mol Catal B:Enz,2006,43(1/4):142-147. |
| [12] | Lu J K,Nie K L,Xie F,et al.Enzymatic Synthesis of Fatty Acid Methyl Esters from Lard with Immobilized Candida sp.99-125[J].Process Biochem,2007,42(9):1367-1370. |
| [13] | Dǒrmǒ N,Bélafi-Bakó K,Bartha L,et al.Manufacture of an Environmental-safe Biolubricant from Fusel Oil by Enzymatic Esterification in Solvent-free System[J].Biochem Eng J,2004,21 (3):229-234. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%