采用微芯片毛细管电泳非接触电导检测法,对黄花鱼中非法添加的工业染料碱性嫩黄O进行了分析.探讨了缓冲液种类、浓度,分离电压和进样时间等因素对分离检测的影响.实验选择5.0 mmol/L乳酸缓冲液(pH =3.29)、1.8 kV分离电压,在1.0 min内实现了碱性嫩黄O的快速分离测定.在优化条件下,碱性嫩黄O浓度的线性范围为5.0 ～ 100.0 mg/L,黄花鱼中碱性嫩黄O的检出限为0.2 mg/kg,该法可成功测定黄花鱼中碱性嫩黄O的含量.
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