在pH =7.4的ris-HCl介质中,利用荧光光谱和紫外吸收光谱法,研究了一种新型蒽环类抗癌药物柔红霉素衍生物(4＇-O-(α-L-夹竹桃糖基)柔红霉素,ODNR)与小牛胸腺DNA(ctDNA)的相互作用.通过离子强度的影响、KI荧光猝灭实验和单双链ctDNA作用的比较实验,分析了ODNR与ctDNA的相互作用模式.结果表明,ODNR通过嵌插方式与ctDNA发生作用.ctDNA对ODNR的荧光有明显的猝灭作用,其机理属于静态猝灭.通过Scatchard方程求得不同温度下的结合常数和结合位点数,由热力学参数确定分子间作用力为疏水作用,也可能存在静电作用.
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