Ni2+是镍基牙科铸造合金中析出的主要金属离子, 是人体的致癌物质. 绿茶的主要活性成分表没食子儿茶素没食子酸酯(EGCG)对包括口腔癌在内的人体癌有化学预防作用. 本研究采用LC/MS法分析EGCG与Ni2+间的相互作用. MTT法检测EGCG、Ni2+及其相互作用对舌鳞癌细胞生长的影响. LC/MS分析显示, Ni2+与EGCG同时加入时有[EGCG-2Ni2+]和[EGCG-Ni2+]形成. Ni2+和EGCG在一定范围内对舌鳞癌细胞生长的抑制呈时间及浓度依赖性. EGCG与Ni2+共同作用后可明显增强对舌鳞癌细胞的抑制作用, 且抑制作用与它们的浓度、加入的顺序及Ni2+与EGCG的比例有关.
Ni2+ is the major ion release from nickel-based dental casting alloy and carcinogens. The major polyphenolic constituent present in green tea, (-)epigallocatechin-3-gallate (EGCG), shows chemoprevention on human cancer. The aim of this study was to determine the interaction between EGCG and Ni2+ and the biological effects on tongue squamous cell carcinoma. The interaction of EGCG and Ni2+ was analyzed by LC/MS. The cell viability was assessed by the MTT reduction assay. The results of LC/MS indicated that [EGCG-2Ni2+] and [EGCG-Ni2+] formed when EGCG and Ni2+ were added simultaneously. Ni2+ and EGCG both inhibited the proliferation of tongue carcinoma cells in a time- and dose-dependent manner. The cytotoxicity against tongue carcinoma cells was increased when EGCG and Ni2+ cotreated, and the inhibition effects were extremely dependent on the concentrations of EGCG and Ni2+, addition order and the ratio of [Ni2+] to [EGCG].
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