利用锂钙硼玻璃在磷酸盐溶液中的原位转化反应制备表面多孔且中空的羟基磷灰石(HA)微球, 将重组人骨形态发生蛋白2(rhBMP-2)装载到微球中, 研究了微球中rhBMP-2的体外缓释行为, 并采用体外细胞培养技术, 将载有rhBMP-2的微球和大鼠骨髓间充质干细胞(MSCs)一起培养, 测定细胞的碱性磷酸酶(ALP)活性, 并与单纯rhBMP-2的作用进行比较. 结果显示, 微球中所装载的rhBMP-2具有明显的缓释效应, 体外释放周期达到1000h以上, 该微球缓释系统具有一定的生物活性, 其作用效果优于单纯使用rhBMP-2.
Hollow hydroxyapatite microspheres consisted of a hollow core and a porous shell were prepared by converting Li2O-CaO-B2O3 glass microspheres in dilute phosphate solution at 37℃. Human bone morphogenetic protein (rhBMP-2) was loaded in the resultant microspheres, and the in vitro release behavior of rhBMP-2 was studied. Furthermore, cell culture technique and ALP kit were used to investigate the effect of the released rhBMP-2 on cell activity. After incubation with rhBMP-2 loaded microspheres, the alkaline phosphatase (ALP) activity of the rat bone marrow mesenchymal stem cells (MSCs) was evaluated. It was found that rhBMP-2 could be released slowly from the hollow HA microspheres for an extended period time up to 1000h. The present delivery system established possessed higher biological activity as compared with pure rhBMP-2.
参考文献
| [1] | Davies S D, Ochs M W. Bone morphogenetic proteins in craniomaxillofacial surgery. Oral Maxillofac Surg. Clin. North Am., 2010, 22(1): 17-31.[2] Dimitriou R, Giannoudis P V. Discovery and development of BMPs. Injury, 2005, 36(3): S28-S33.[3] Fei Z Q, Hu Y Y, Wu D C, et al. Preparation and property of a novel graft composite consisting of rhBMP-2 loaded PLGA microspheres and calcium phosphate cement. J. Mater. Sci: Mater. Med., 2008, 19(3): 1109-1116.[4] Liu H N, Yazici H L, Ergun C D, et al. An in vitro evalution of the Ca/P ratio for the cytocompatibility of nano-to-micron particulate calcium phosphates for bone regeneration. Acta Biomater., 2008, 4(5): 1472-1479.[5] 杨为中, 周大利, 尹光福, 等(YANG Wei-Zhong, et al). 磷酸钙/ BMP复合生物活性骨水泥水化性能及诱导成骨特性研究. 无机材料学报(Journal of Inorganic Materials), 2005, 20(5): 1174-1180.[6] Palazzo M C, Sidoti N, Roveri A, et al. Controlled drug delivery from porous hydroxyapatite grafts: an experimental and theoretical approach. Mater. Sci. Eng. C, 2005, 25(2): 207-213.[7] Matsumoto T, Okazaki M, Inoue M, et al. Hydroxapatite particles as a controlled release carrier of protein. Biomaterials, 2004, 25(17): 3807-3812.[8] Schnettler R, Alt V, Dingeldein E, et al. Bone ingrowth in bFGF-coated hydroxyapatite ceramic implants. Biomaterials, 2003, 24(25): 4603-4608.[9] 姚爱华, 艾凡荣, 刘 欣, 等(YAO Ai-Hua, et al). 硼酸盐玻璃转化制备中空羟基磷灰石微球的研究. 无机材料学报(Journal of Inorganic Materials), 2010, 25(1): 53-57.[10] Yao A H, Ai F R, Liu Xin, et al. Preparation of hollow hydroxyapatite microspheres by the conversion of borate glass at near room temperature. Mater. Res. Bull., 2010, 45(1): 25-28.[11] 季晓风, 丛宪玲, 杨晓玉, 等. 人骨形成蛋白纳米微粒的制备与生物活性鉴定. 中国实验诊断学, 2006, 10(3): 261-263.[12] Tanaka H, Mizokami H, Shiigi E, et al. Effects of basic fibroblast growth factor on the repair of large osteochondral defects of articular cartilage in rabbits: dose-response effects and long-term outcomes. Tissue Eng., 2004, 10(3/4): 633-641.[13] Nakashima M, Reddi A H. The application of bone morphogenetic proteins to dental tissue engineering. Nat. Biotechnol., 2003, 21(9): 1025-1032.[14] Hu Y Y, Zhang C, Zhang S M, et al. Development of a porous poly(L-lactic acid)/hydroxyapatite/ collagen scaffold as a BMP delivery system and its use in healing canine segmental bone defect. J. Biomed. Mater. Res. A, 2003, 67(2): 591-598. |
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