以常规马弗炉和微波烧结两种方式分别制备羟基磷灰石陶瓷颗粒, 对其相组成、微观形貌、表面zeta电位以及蛋白吸附行为进行分析对比. 结果显示, 尽管两种烧结方式制备的羟基磷灰石陶瓷颗粒具有相同的相组成, 但是它们的微观结构、表面zeta电位和蛋白吸附行为存在明显差异. 相对于常规烧结, 微波烧结得到的颗粒具有丰富的微孔隙和接近于纳米尺度的晶粒尺寸, 其表面zeta电位值更小, 能够吸附更多的牛血清白蛋白和更少的溶菌酶. 微波烧结在制备高生物活性纳米羟基磷灰石陶瓷上可能是一种较为理想的方法.
Two types of hydroxyapatite (HA) ceramic particles were respectively fabricated by conventional and microwave sintering processes. The conventionally sintered HA was abbreviated as HACS, and the microwave sintered one was HAMS. The phase compositions, surface morphologies and zeta potentials of both particles were respectively analyzed with X-ray diffraction (XRD), scanning electron microscope (SEM) and zetasizer. Bovine serum albumin (BSA) and lysozyme (LSZ) were selected as models to study their adsorption behaviors on HACS and HAMS. Results confirm that both HA particles crystallize completely, but HACS has larger crystal grain size than HAMS. Although both HACS and HAMS show negative surface zeta potentials in phosphate buffered saline (PBS, pH 7.4), the former has higher absolute value than the latter. Besides, both HA particles exhibit different adsorption ability for BSA and LSZ, and HACS adsorbs fewer BSA but more LSZ than HAMS. The microwave sintering can be a good method to produce nano-HA ceramics with excellent bioactivity.
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