建立了气相色谱-质谱法测定牛奶中三聚氰胺和三聚氰酸含量的分析方法.样品经二乙胺-乙腈-水溶液超声提取,离心,滤液用氮气吹干后加入硅烷化衍生试剂衍生,气相色谱-质谱测定,选择离子模式监测,外标法定量.在0.025～2 mg/kg范围内,目标物的峰面积与其质量浓度的线性关系良好(r>0.98);在0.5,1.0和2.5 mg/kg的添加水平,三聚氰胺和三聚氰酸的平均回收率分别为84% ～87%和75% ～102% ,相对标准偏差(RSD)分别为5.7% ～11.7%和4.9% ～7.8% ;三聚氰胺和三聚氰酸的检出限(LOD)分别为0.05 mg/kg和0.10 mg/kg.结果表明:该方法简便、快速、灵敏、准确,适合牛奶中三聚氰胺和三聚氰酸的确证和定量测定.
参考文献
| [1] | Yokley R A,Mayer L C,Rezaaiyan R,et al.J Agric Food Chem,2000,48:3352 |
| [2] | Sancho J V,Ibaez M,Grimalt S,et al.Anal Chim Acta,2005,530:237 |
| [3] | 中华人民共和国卫生部、工业和信息化部、农业部、国家工商行政管理总局、国家质量监督检验检疫总局.中华人民共和国卫生部、工业和信息化部、农业部、国家工商行政管理总局、国家质量监督检验检疫总局公告:2008年第25号.Ministry of Health,Ministry of Industry and information Technology,Ministry of Agriculture,State Administration for Industry and Commerce,General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China.Bulletin issued by Ministry of Health,Ministry of Industry and information Technology,Ministry of Agriculture,State Administration for Industry and Commerce,General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China:No.25,2008.[2008-10-08].http:/ /www.aqsiq.gov.cn/zjxw/zjxw/zjftpxw/200810/t20081008_92610.htm |
| [4] | Cantu R,Evans O,Magnuson M L.Chromatographia,2001,53(7/8):454 |
| [5] | Puschner B,Poppenga R H,Lowenstine L J,et al.J Vet Diagn Invest,2007,19(6):616 |
| [6] | WHO.Melamine and cyanuric acid:toxicity,preliminary risk assessment and guidance on levels in food.[2008-09-25].http:/ /www.who.int/entity/foodsafety/fs_management/infosan_events/en/index.html |
| [7] | Reimschuessel R,Gieseker C M,Miller R A,et al.AJVR,2008,69(9):1217 |
| [8] | Brown C A,Jeong K S,Poppenga R H,et al.J Vet Diagn Invest,2007,19:525 |
| [9] | Ehling S,Tefera S,Ho I P.Food Addit Contam,2007,24(12):1319 |
| [10] | FDA.GC-MS screen for the presence of melamine and cyanuric acid.[2007-05-07].http:/ /www.fda.gov/cvm/GCMSMelamine.htm |
| [11] | Ding T,Xu J Z,Li J Z,et al.Chinese Journal of Chromatography (丁涛,徐锦忠,李建忠,等.色谱),2008,26(1):6 |
| [12] | Cai Q R,Ouyang Y Y,Qian Z J,et al.Chinese Journal of Chromatography (蔡勤仁,欧阳颖瑜,钱振杰,等.色谱),2008,26(3):339 |
| [13] | Filigenzi M S,Puschner B,Aston L S.J Agric Food Chem,2008,56(17):7593 |
| [14] | GB/T 22388 |
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