采用反相高效液相色谱法测定了枇杷叶中科罗索酸的含量.将样品在90%乙醇溶液中于90 ℃下提取3.0 h,提取3次,浓缩溶液后加水沉淀,将沉淀溶解于甲醇溶液后在反相色谱柱上分离,以甲醇-1.0%醋酸水溶液(体积比为88∶12)为流动相,流速0.8 mL/min,在波长215 nm处进行测定.结果表明:安徽黄山产枇杷叶中科罗索酸含量(以质量分数计)为0.36%,科罗索酸平均加标回收率为99%.该方法方便快速,结果准确可靠.
参考文献
| [1] | Judy W V,Hari S P,Stogsdill W W,Judy J S,Naguib Y M A,Passwater R.J Ethnopharmacol,2003,87:115 |
| [2] | Taniguchi S,Imayoshi Y,Kobayashi E,Takamatsu Y,Ito H,Hatano T,Sakagami H,Tokuda H,Nishino H,Sugita D,et al.Phytochemistry,2002,59:315 |
| [3] | A Natural Approach to Lowering Blood Sugar.Life Extension Magazine,2000,September[2005-07-10].http://www.lef.org/magazine/mag2000/sep2000_report_blood.html |
| [4] | Ju Jianhua,Zhou Liang,Lin Geng,Liu Dong,Wang Liwei,Yang Junshan.Chinese Pharmaceutical Journal (鞠建华,周亮,林耕,刘东,王立为,杨峻山.中国药学杂志),2003,38(10):752 |
| [5] | Luo Xiaoqing,Guo Xiaoyi,Yu Xuewei.Chinese Wild Plant Resources (罗晓清,郭小仪,俞学炜.中国野生植物资源),2004,23(5):50 |
| [6] | Tang Liqin,Liu Sheng,Li Chu,Chen Liming.Chinese Journal of Hospital Pharmacy (唐丽琴,刘圣,李矗,陈礼明.中国医院药学杂志),2004,24(12):725 |
| [7] | Ren Xiulian,Wei Qifeng,Zhou Chunshan,Zhong Shian,Dong Chaoqing.Journal of Central South University of Technology:Science and Technology (任秀莲,魏琦峰,周春山,钟世安,董朝青.中南大学学报:自然科学版),2004,35(4):599 |
| [8] | Oxford University.Safety (MSDS) Data for Corosolic Acid.(2005-02).http://physchem.ox.ac.uk/MSDS/CO/corosolic_acid.html |
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