建立了反相高效液相色谱-蒸发光散射检测器(RP-HPLC-ELSD)测定紫苏子油中α-亚麻酸含量的方法.紫苏子油样用0.5 mol/L的KOH-CH3OH溶液于60℃水浴中皂化20 min、6 mol/L的盐酸酸化后用无水乙醚萃取,再用氮气流吹干乙醚,用甲醇溶解并定容,然后直接进行RP-HPLC-ELSD分析.结果表明,当α-亚麻酸质量为6.2～45.4μg时,其峰面积A的对数(lg A)与α-亚麻酸质量m(μg)的对数(lg m)呈线性关系,线性方程为lg A=1.367 5·lg m-1.628,相关系数为0.997 3(n=5).以信噪比S/N=3计算,方法的检出限为0.11μg.被分析的紫苏子油中α-亚麻酸的含量为6.79%(质量分数),相对标准偏差(RSD)为5.1%(n=5);平均回收率为102%,RSD为6.3%(n=5).该方法简单、快速、准确.
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