由于纯水在高温高压下形成亚临界液体水时氢键网络发生改变,导致其极性、粘度等物理性质产生较大的变化.以亚临界水为流动相的高效液相色谱方法(SubWC)是近年来发展起来的一种新型分离技术.SubWC的仪器系统可以采用通过改装的一般的气相色谱(GC)或液相色谱(LC)装置;分离色谱柱既可以采用液相色谱填充柱,也可以采用类似于GC的毛细管柱;选择性既可以通过调节柱系统的温度和压力,也可以通过在流动相中添加有机调节剂或盐类进行调节;检测既可用氢火焰离子化检测器检测,也可用紫外检测器检测,极大地拓宽了色谱分离和最佳条件选择的范围.SubWC无论在仪器系统、流动相的洗脱还是固定相的选择等方面均有一定的特征.这种新的分离模式目前尚处于研究与开发阶段,且多用于极性、中等极性样品的快速分离.
参考文献
| [1] | Antia F D, Horvath C. J Chromatogr, 1988, 435(1): 1 |
| [2] | Han J-A,LimS-T. Carbohydrate Polymers, 2004, 55(3): 265 |
| [3] | Silva-Lucca R A,Tabak M,Nascimento O R,Roque-Barreira M C,Beltramini L M. Biophys Chem, 1999, 79(2): 81 |
| [4] | Cirilli R, Costi R,Di Santo R, Artico M, Roux A, Gallinella B, Zanitti L,La Torre F. J Chromatogr A, 2003, 993: 17 |
| [5] | Zhou L,Thompson R,Reamer R A,Miller C,Welch C,Ellison D K,Wyvratt J M. J Chromatogr A, 2003, 987: 409 |
| [6] | Miller D J, Hawthorne S B. Anal Chem, 1997, 69(4): 623 |
| [7] | Blackwell J A, Stringham R W, Weckwerth J D. Anal Chem, 1997, 69(3): 409 |
| [8] | Smith R M, Burgess R J. J Chromatogr A, 1997, 785: 49 |
| [9] | Greibrokk T, Andersen T. J Chromatogr A, 2003, 1 000(1-2): 743 |
| [10] | Yang Y, Li B. Anal Chem, 1999, 71(8): 1 491 |
| [11] | Yang Y, Lamm L J, He P, Kondo T. J Chromatogr Sci, 2002, 40(2): 107 |
| [12] | Yang Y, Jones A D, Mathis J A, Francis M A. J Chromatogr A, 2002, 942(1-2): 231 |
| [13] | Yang Y. In: Abstracts of Papers for 223rd ACS National Meeting,Orlando, USA, April 7-11, 2002. IEC-277 |
| [14] | Kondo T, Yang Y, Lamm L. Anal Chim Acta, 2002, 460(2): 185 |
| [15] | He P, Yang Y. J Chromatogr A, 2003, 989: 55 |
| [16] | LammL J, Yang Y. Anal Chem, 2003, 75(10): 2 237 |
| [17] | Li B, Yang Y, Gan Y, Eaton C D, He P, Jones A D. J Chromatogr A, 2000, 873: 175 |
| [18] | Jones A, Yang Y. Anal Chim Acta, 2003, 485(1): 51 |
| [19] | Yang Y, Jones A D, Eaton C D. Anal Chem, 1999, 71(17): 3 808 |
| [20] | Kondo T, Yang Y. Anal Chim Acta, 2003, 494(1-2): 157 |
| [21] | Liu Guanghui. Natural Gas Chemical Industry (刘光会. 天然气化工), 2000, 25(6): 46 |
| [22] | Wu Renming.Progress in Chemistry (吴仁铭. 化学进展), 2002, 14(1): 32 |
| [23] | Han Hai, Yu Rongmin, Wang Huan, Sun Pinghua. Journal of Pediatric Pharmacy (韩海, 于荣敏,王欢, 孙平华. 儿科药学杂志), 2003, 9(4): 11 |
| [24] | Renn C N, Synovec R E. J Chromatogr, 1991, 536: 289 |
| [25] | Lima L R, Synovec R E. Anal Chem, 1993, 65(2): 128 |
| [26] | Liu G, Svenson L, Djordjevic N, Erni F. J Chromatogr, 1993, 633: 25 |
| [27] | Li Ling, Lu Feng, Sun Peng, Yuan Yongfang, Wu Yutian. Acta Pharmaceutica Sinica (李玲,陆峰, 孙鹏, 原永芳, 吴玉田. 药学学报), 2000, 35(11): 832 |
| [28] | Lu F, Li L, Sun P, Wu Y T. Journal of Chinese Pharmaceutical Science, 2001, 10(1): 39 |
| [29] | Ingelse B A, Janssen H G, Cramers C A. J High Resolut Chromatogr, 1998, 21(11): 613 |
| [30] | Scott K T, Dasgupta P D. Talanta, 2002, 56(6): 977 |
| [31] | Pawlowski T M, PooleC F. Anal Commun, 1999, 36(3): 71 |
| [32] | Lü Xiuyang, He Long, Zheng Zansheng, Cai Lei. Chemical Industry and Engineering Progress (吕秀阳, 何龙, 郑赞胜, 蔡磊. 化工进展), 2003, 22(5): 477 |
| [33] | Miller D J, Hawthorne S B. Anal Chem, 1998, 70(8): 1 618 |
| [34] | Coym J W,Dorsey J G. J Chromatogr A, 2004, 1 035: 23 |
| [35] | Smith R M, Burgess R J. Anal Commun, 1996, 33(7): 327 |
| [36] | Zhao J H, Carr P W. Anal Chem, 2000, 72(2): 302 |
| [37] | Kephart T S, Dasgupta P K. Anal Chim Acta, 2000, 414(1-2): 71 |
| [38] | Bruckner C A, Ecker S T, Synovec R E. Anal Chem, 1997, 69(17): 3 465 |
| [39] | Marin S J,Jones B A,Felix W D,Clark J. J Chromatogr A, 2004, 1 030: 255 |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%