建立了一个快速、简单、准确的固相萃取和高效液相色谱相结合的测定苦瓜甙A含量的方法。样品经石墨碳固相萃取管（3 mL/250 mg）纯化后以高效液相色谱检测。色谱柱为C18，流动相为V(乙腈)∶V(甲醇)∶V(50 mmol/L磷酸二氢钾缓冲液)=25∶20∶60，流速为0.8 mL/min，检测波长为208 nm。标准曲线自10 mg/L到1*!000 mg/L呈线形关系（r2=0.999*!2）。该方法具有很好的重现性，日内或日间的相对标准偏差和相对平均误差均小于10%。样品回收率大于90%。
A rapid, simple and accurate method for the determination of momordicoside A has been established using solid phase extraction (SPE) followed by high performance liquid chromatography (HPLC). Sample was processed by SPE on a Carb cartridge (3 mL/250 mg), and was then determined by HPLC on a C18 column (4.6 mm i.d.×250 mm, 5 μm) with V(acetonitrile)∶V(methanol)∶V(50 mmol/L potassium dihydrogen phosphate buffer)=25∶20∶60 as mobile phase (0.8 mL/min) and UV detection at 208 nm. The calibration curves were linear from 10 mg/L to 1*!000 mg/L (r2=0.999 2). The analytical method was shown to be highly reproducible, giving all of the relative standard deviations and relative mean errors less than 10% for both intra-day and inter-day determinations. The absolute recoveries were greater than 90%.
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