采用反相高效液相色谱法，一次进样、同时测定食品中的人工合成甜味剂（糖精钠、安赛蜜、甜味素）、防腐剂（苯甲酸、山梨酸）、咖啡因、可可碱和茶碱。以Alltech Econosphere C18柱（150 mm×4.6 mm i.d.，3 μm）为分离柱，10 mmol/L NaH2PO4 (pH 4.00)-乙腈（体积比为90∶10）为流动相，采用二极管阵列检测器进行检测。整个分离过程在23 min内完成。样品平均加标回收率为78.5%～107.2%。方法也可用于药品分析。
A novel method is proposed for the simultaneous separation and determination of eight food additives, acesulfame potassium (AK), aspartame (ASP), benzoic acid (BA), caffeine (CA), saccharin sodium (SA), sorbic acid (SOR), theobromine (TB) and theophylline (TP) by reversed-phase high performance liquid chromatography． The separation was achieved within 23 min by using an Alltech Econosphere C18 column with 10 mmol/L NaH2PO4 (pH 4．00)-acetonitrile (90∶10,V/V) as mobile phase. The qualification and quantitation were accomplished by using a photodiode array detector． The detection limits (S/N=3) for all analytes were below mg/L level． Under the experimental conditions, other common food additives and organic acids such as cyclamate, citric acid, malic acid, tartaric acid and ascorbic acid, did not interfere with the determination． The method has been successfully applied to the analysis of various foods as well as pharmaceutical preparation, and the average recoveries for real samples ranged from 78．5% to 107．2%．
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