利用高效液相色谱法分析测定青霉素裂解液中的主要成分6-氨基青霉烷酸(6-APA)、青霉素G钾和苯乙酸，并对副产物青霉噻唑酸进行了液相色谱的定性检测。结果表明，实验方法简便、快速，结果准确，可以用于6-APA生产的质量控制。
A method for the determination of main composition in degradedproducts of penicillin, 6-aminopenicillanic acid (6-APA),penicillin G ka lium(PGK), phenylacetic acid(PAA), and the by-product benzylpenicilloic acid(BP A) by HPLC was studied. Separation conditions were as follows: Spherisorb C18 column, 250 mm×4.6 mm i.d., 10 μm; mobile phase: V(methanol)∶V (0.004 mol/L KH2PO4 buffer, pH 4.5)=50∶50; flow rate: 1 mL/min; detector: UV 230 nm. The method is effective, quick, accurate and reproducible. The satis factory results show that this new method has certain practical values as an approach of quality control.
参考文献
| [1] | WU Run-ling, CAI Shun-xin. Chinese Journal of Anti-biotics, 1996, 21(3):234-236吴润玲,蔡顺鑫. 中国抗生素杂志, 1996, 21(3):234-236 |
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| [3] | Adlaed M W, Gordon B M, Keshavarz T, et al. Biotechnol Tech, 1991 , 5(2):121-126 |
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