目的：研究海洋环境下高压脉冲电场对微生物污损的抑制效果和机理。方法采用刷涂法制备碳纤维/环氧防腐涂料复合涂层，研究碳纤维长度和含量对复合涂层的介电性能和表面能的影响以及碳纤维复合涂层在高压脉冲电场作用下的杀菌性能。结果高频电场下，碳纤维长度和含量是影响复合涂层介电性能的主要因素，随着电场强度、频率在设定范围内增加，涂层的杀菌率显著增大；而随占空比的增大，涂层的杀菌率呈先增后减最后趋于平缓的趋势，占空比为0.5时涂层的杀菌率最高达99.8％。结论掺杂0.1％(质量分数)5 mm碳纤维的复合涂层介电性能优良。高压脉冲电场对细菌具有良好杀灭效果，涂层的杀菌率最高可达99.97％。
ABSTRACT:Objective To explore the inhibition effects and mechanism of high-voltage pulsed electric field ( HPEF) in marine environment on microbial fouling. Methods Carbon fiber / epoxy composite anti-corrosion coating was prepared by brushing meth-od, and the effects of the carbon fiber length and content on the dielectric properties and surface energy of the composite coating as well as the bactericidal performance of the carbon fiber composite coating in high-voltage pulsed electric field. Results The results showed that the carbon fiber length and content were the main factors affecting the dielectric properties of the composite coating un-der high frequency. With the increase of electric field intensity and frequency within the set range, the sterilization rate of the coat-ing obviously increased, while with the increase of duty cycle, the sterilization rate of the coating first increased and then decreased and finally tended to be stable. When the duty cycle was 0. 5, the maximum sterilization rate of 99. 8% was reached. Conclusion The composite coating with a doping content of 0. 1% 5 mm carbon fiber had excellent dielectric properties. High-voltage pulsed e-lectric field had a good killing effect on bacteria and the sterilization rate of the coating could reach 99. 97%.
参考文献
| [1] | Wen Jing Yang;Koon-Gee Neoh;En-Tang Kang .Polymer brush coatings for combating marine biofouling[J].Progress in Polymer Science,2014(5):1017-1042. |
| [2] | KAMINO K .Barnacle Adhesives and Adhesion[J].Biofoul-ing,2013,29(6):735-749. |
| [3] | The Adhesive Strategies Of Cyprids And Development Of Barnacle-resistant Marine Coatings[J].Biofouling,2008(5/6):p.351-363. |
| [4] | Jian-Wen Qiu;Vengatesen Thiyagarajan;Sam Cheung;Pei-Yuan Qian .Toxic effects of copper on larval development of the barnacle Balanus amphitrite[J].Marine pollution bulletin,2005(8/12):688-693. |
| [5] | Yebra DM;Kiil S;Dam-Johansen K .Antifouling technology - past, present and future steps towards efficient and environmentally friendly antifouling coatings[J].Progress in Organic Coatings: An International Review Journal,2004(2):75-104. |
| [6] | Almeida E;Diamantino TC;de Sousa O .Marine paints: The particular case of antifouling paints[J].Progress in Organic Coatings: An International Review Journal,2007(1):2-20. |
| [7] | 钱鑫,支建海,王雪飞,张永刚,杨建行.碳纤维表面结构对复合材料吸湿性能的影响[J].无机材料学报,2013(02):189-194. |
| [8] | 伏金刚,朱冬梅,周万城,罗发.定向分布碳纤维复合材料介电性能研究[J].无机材料学报,2012(11):1223-1227. |
| [9] | N.I. Lebovka;H. Mhemdi;N. Grimi;O. Bals;E. Vorobiev .Treatment of potato tissue by pulsed electric fields with time-variable strength: Theoretical and experimental analysis[J].Journal of food engineering,2014(Sep.):23-31. |
| [10] | ANGERSBACH A;HEINZ V;KNORR D.Effects of Pulsed Electric Fields on Cell Membranes in Real Food Systems[J].Innovative Food Science,Emerging Technologies,2001(2):135-149. |
| [11] | Caminiti, I. M;Palgan, I;Noci, F;Munoz, A;Whyte, P;Cronin, D. A;Morgan, D. J;Lyng, J. G. .The effect of pulsed electric fields (PEF) in combination with high intensity light pulses (HILP) on <i>Escherichia coli</i> inactivation and quality attributes in apple juice.[J].Innovative Food Science & Emerging Technologies,2011(2):118-123. |
| [12] | 刘新雨,曾新安,贾晓.脉冲电场对荔枝汁中酿酒酵母的杀灭效果[J].食品科学,2011(03):91-94. |
| [13] | Jean-Marie Vallerot;Xavier Bourrat;Arnaud Mouchon .Quantitative structural and textural assessment of laminar pyrocarbons through Raman spectroscopy, electron diffraction and few other techniques[J].Carbon: An International Journal Sponsored by the American Carbon Society,2006(9):1833-1844. |
| [14] | C. Hellio;D. De La Broise;L. Dufosse .Inhibition of marine bacteria by extracts of macroalgae: potential use for environmentally friendly antifouling paints[J].Marine environmental research,2001(3):231-247. |
| [15] | Isla Fitridge;Tim Dempster;Jana Guenther;Rocky de Nys .The impact and control of biofouling in marine aquaculture: a review[J].Biofouling,2012(7/8):649-669. |
| [16] | ZIMMERMANN U;ARNOLD W M;MEHRLE W .Biophys-ics of Electroinjection and Electrofusion[J].Journal of E-lectrostatics,1988,21(2/3):309-345. |
| [17] | TSONG T Y .Electroporation of Cell Membranes[J].Bio-physical Journal,1991,60(2):297-306. |
| [18] | El Zakhem H;Lanoiseille JL;Lebovka NI;Nonus M;Vorobiev E .The early stages of Saccharomyces cerevisiae yeast suspensions damage in moderate pulsed electric fields[J].Colloids and Surfaces, B. Biointerfaces,2006(2):189-197. |
| [19] | Jaeger, H.;Meneses, N.;Knorr, D. .Impact of PEF treatment inhomogeneity such as electric field distribution, flow characteristics and temperature effects on the inactivation of E. coli and milk alkaline phosphatase.[J].Innovative Food Science & Emerging Technologies,2009(4):470-480. |
| [20] | SERGEY DOBRETSOV;HANS-UWE DAHMS;PERI-YUAN QIAN .Inhibition of biofouling by marine microorganisms and their metabolites[J].Biofouling,2006(1/2):43-54. |
| [21] | Garner AL;Chen G;Chen N;Sridhara V;Kolb JF;Swanson RJ;Beebe SJ;Joshi RP;Schoenbach KH .Ultrashort electric pulse induced changes in cellular dielectric properties[J].Biochemical and Biophysical Research Communications,2007(1):139-144. |
| [22] | Raimonda Celiesiute;Romualdas Trusovas;Gediminas Niaura;Vitas Svedas;Gediminas Raciukaitis;Zivile Ruzele;Rasa Pauliukaite.Influence of the laser irradiation on the electrochemical and spectroscopic peculiarities of graphene-chitosan composite film[J].Electrochimica Acta,2014:265-276. |
| [23] | Heinz V;Alvarez I;Angersbach A;Knorr D .Preservation of liquid foods by high intensity pulsed electric fields - basic concepts for process design[J].Trends in food science & technology,2001(3/4):103-111. |
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