羟基磷灰石因有良好的生物学活性被广泛应用于人工骨替代材料,但纯羟基磷灰石存在的自身不足限制了其临床应用范围.人体硬组织中含有多种具有重要生化作用的微量元素,本研究按照骨骼中微量元素含量对羟基磷灰石进行掺杂改性,制备出一种新型仿骨成分多元掺杂纳米结构羟基磷灰石材料(HA2).通过碱性磷酸酶活性染色、半定量PCR测量和茜素红染色等不同方法对比了未掺杂纳米结构羟基磷灰石(HA1)和多元掺杂纳米结构羟基磷灰石材料(HA2)对小鼠成骨前体细胞(MC3T3-E1)增殖及分化的影响.碱性磷酸酶活性染色实验证明HA2比HA1具有更强的促进细胞产生ALP的作用;半定量PCR测量证明HA2比HA1对成骨基因的表达有更为显著的促进作用;茜素红对矿化结节的染色实验同样证明HA2比HA1更有利于成骨细胞发生矿化.相对于未掺杂纳米结构HA1,多元掺杂纳米结构HA2对小鼠成骨前体细胞MC3T3-E1的分化及成骨有显著促进作用.多元掺杂改性使人工合成纳米结构羟基磷灰石更接近人体骨的无机成分,具有优良的成骨性能,是一种有前途的硬组织植入体材料.
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