材料期刊网

为构建一种诱导型骨修复支架材料,将淫羊藿苷(Ica)经过氨基化改性得到 Ica-NH2,并对 Ica-NH2进行 FTIR、XRD和TG-DTA表征。以 Ica-NH2共价结合壳聚糖(CS)为诱导因子添加方式,聚羟基丁酸酯-羟基戊酸酯(PHBV)和CS为基材,经过两相混合急速冷冻/冷冻干燥成型技术制备了 Ica-NH2-CS/PHBV骨组织工程支架。随后,对支架材料进行了 SEM、体外药物缓释、力学性能、细胞相容性及细胞增殖评价。结果显示：氨基化改性 Ica的 FTIR谱图在3371、3328 cm-1处出现2个中等强度、尖锐的 N—H 伸缩振动峰,在1689 cm-1处出现N—H 变角振动峰；XRD谱图显示其衍射增强且整体左移,证明 Ica被成功氨基化改性；支架材料显微结构呈网络状串珠状,并均匀分布着3~10μm的微孔,其体外药物缓释效果良好,力学强度介于硬质骨与松质骨之间,复合细胞培养7 d 后表现出良好的贴附与增殖,且细胞在 Ica-NH2-CS/PHBV支架材料上的增殖增长率显著高于 CS/PHBV。研究表明所构建的 Ica-NH2-CS/PHBV支架材料可作为一种良好的诱导型骨修复材料。

In order to fabricate an inducible bone repair scaffold materials,Icariin (Ica)were modified by amino and then got Ica-NH2 ,Ica-NH2 was characterized by FTIR,XRD and TGA.Ica-NH2 covalent binding with carboxym-ethyl chitosan (CS)as the inducing factor addition method,poly hydroxybutyrate-co-hydroxyvalerate (PHBV)and CS were used as basic materials,then two-phase mixed rapid freezing/freeze-drying technology was used to build the Ica-NH2-CS/PHBV bone tissue engineering scaffolds.And then the scaffold materials were evaluated by SEM,in-vitro drug release,mechanical properties,cell compatibility and proliferation.The results show that:there were two moderate intensity,sharp N—H stretching vibration peaks at 3 371 and 3 328 cm-1 in the FTIR spectra of ami-nated Ica,one N—H waving vibration peaks appears at 1 689 cm-1 ,XRD pattern shows the diffractions are en-hanced and move to the left as a whole,these results indicate that Ica is successfully amino-modified.The scaffold materials possess network-and bead-like mircostructures,which are uniformly distrubuted wtih microporous of 3-10μm.Ica-NH2-CS/PHBV scaffold has good invitro drug release effect.Mechanical strength tests show its me-chanical strength is between dense bone and cancellous bone,composite cells closely adhere on the scaffold after 7 d cultivation and proliferate well,and the proliferation rate of cells attached on Ica-NH2-CS/PHBV scaffold materials is significantly higher than that on the CS/PHBV.This research indicates that Ica-NH2-CS/PHBV scaffold materi-als could be used as good type of inducible bone repair materials.