基于适配子构建了无标记检测三磷酸腺苷(ATP)的微悬臂梁生物传感器.将ATP适配子修饰在微悬臂梁阵列中的传感悬臂镀金面上,用来识别ATP,而参比悬臂修饰巯基己醇(MCH)防止非特异性吸附.ATP与其适配子发生特异性相互作用,使悬臂的上下两个表面产生应力差,导致传感悬臂产生偏转,扣除参比悬臂偏转后其偏转值与ATP的浓度在0.5～5 mmol/L范围内有良好的线性关系,相关系数为0.998,最低检出限为0.06 mmol/L.该微悬臂梁生物传感器响应快速、操作简单,并且对ATP具有良好的特异性.
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