本研究以拟南芥(Columbia野生型)干种子为材料,利用兰州重离子研究装置(HIRFL)产生的碳离子束对材料进行辐射处理,统计其存活率、根长、下胚轴长及每果荚种子数,以探讨不同传能线密度(Linear Energy Transfer,LET)的碳离子束辐照对拟南芥当代损伤效应的影响.结果表明,在相同LET辐射条件下,随着辐射剂量的增大,拟南芥的存活率、根长、下胚轴长度、每果荚种子数都呈现下降趋势.在相同剂量不同LET辐射处理情况下,随着LET的增大,存活率、根长、下胚轴长、每果荚种子数都显著下降,可见高LET辐射严重抑制了拟南芥的生长和发育.研究表明,当LET为50 keV/μm时,碳离子束辐射拟南芥干种子对应的最佳诱变剂量为200 Gy,为后续开展碳离子束辐射的诱变效率研究奠定了前期基础.
Aimed to study the biological effects of carbon ion beams with different linear energy transfer (LET) values provided by Heavy Ion Research Facility in Lanzhou (HIRFL),dry seeds of Arabidopsis thaliana (Columbia-WT) were irradiated and a series of biological effects of postembryonic development,such as survival rate,primary root length,hypocotyls length and number of seeds per silique,were investigated.The results showed that,under the radiation condition of the same LET value,the survival rate,root length,hypocotyls length and number of seeds per silique were decreased with the increasing dose.In addition,under the radiation conditions with different LET values,but same dose,the extent of the decline of the survival rate,root length,hypocotyls length and number of seeds per silique were reinforced with the increasing LET.It was also found that high LET radiations inhibited the subsequent growth and development of Arabidopsis thaliana severely.In brief,it was suggested that the optimum dose of carbon ion beam with 50 keV/μm value on Arabidopsis thaliana dry seeds was 200 Gy.This research complemented the preliminary theoretical foundation for the comparative study of the highest mutation efficiency of carbon ion beam irradiations at IMP,CAS(Institute of Modern Physics,Chinese Academy of Sciences).
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