报告-种氨基功能化处理的多壁碳纳米管(f-MWCNTs)用于生物分子[如牛血浆蛋白素(BSA)蛋白质及脱氧核醣核酸(DNA)]的接枝处理高效方法.以苯-二茂铁喷入氩氛炉于～850 ℃裂解制得MWCNTs,,而后向纳米管表面导入羧基化合物,再以氨基基团对MWCNTs,进行功能化处理.该过程包括:乙二胺与羧基基团直接结合,经酰胺化引入胺基基团.对制得的MWCNTs、 f-MWCNTs以及由BSA蛋白质和DNA接枝的氨化f-MWCNTs等样品,采用SEM、TEM、FTIR进行表征.结果表明:生物分子(BSA蛋白质和DNA)接枝于氨化的f-MWCNTs.
An efficient method for the attachment of biomolecules [e.g. bovine serum albumin (BSA) protein and deoxyribonucleic acid (DNA)] to amino-group-functionalized multiwalled carbon nanotubes (f-MWCNTs) was reported. MWCNTs were prepared by spray pyrolysis of a benzene-ferrocene solution in argon atmosphere at ～850 ℃ followed by functionalization with an amino group by chemical modification of carboxylic groups introduced on the nanotube surface. This process involves a direct coupling of ethylenediamine with carboxylic groups to introduce amino groups by amide formation. The as-synthesized MWCNTs, f-MWCNTs, and amino f-MWCNTs with BSA protein and DNA were characterized by scanning and transmission electron microscopy, and Fourier transform infrared spectroscopy, which confirm the attachment of biomolecules (BSA protein and DNA) to the amino f-MWCNTs.
参考文献
| [1] | Mauricio Terrones .SCIENCE AND TECHNOLOGY OF THE TWENTY-FIRST CENTURY: Synthesis, Properties, and Applications of Carbon Nanotubes[J].Annual review of materials research,2003(0):419-501. |
| [2] | Sinha N.;Yeow J.T.-W. .Carbon nanotubes for biomedical applications[J].IEEE transactions on nanobioscience,2005(2):180-195. |
| [3] | Bianco A;Kostarelos K;Partidos C D.Biomedical applications of functionalized carbon nanotubes[J].Chemical Communications,2005:571-577. |
| [4] | Hu H;Ni Y;Montana V et al.Chemically functionalized carbon nanotubes as substrates for neuronal growth[J].Nano Letters,2004,4:507-5511. |
| [5] | Pantarotto D;Singh R;McCarthy D et al.Functionalized carbon nanotubes for plasmid DNA gene delivery[J].Angewandte Chemie International Edition,2004,43:5242-55246. |
| [6] | Alberto Bianco;Johan Hoebeke;Sylvie Godefroy;Olivier Chaloin .Cationic Carbon Nanotubes Bind to CpG Oligodeoxynucleotides and Enhance Their Immunostimulatory Properties[J].Journal of the American Chemical Society,2005(1):58-59. |
| [7] | Wang S G;Wang R;Sellin P J et al.DNA boisensors based on self-assembled carbon nanotubes.Biochem[J].Biophysical Research Communications,2004,325:1433-1437. |
| [8] | Nadine Wogn Shi kam;Theodore C.Jessop;Paul A.Wender;Hongie Dai .Nanotube Molecular Transporters: Internalization of Carbon Nanotube-Protein Conjugates into Mammalian Cells[J].Journal of the American Chemical Society,2004(22):6850-6851. |
| [9] | Yu L;Li CM;Zhou Q;Gan Y;Bao QL .Functionalized multi-walled carbon nanotubes as affinity ligands - art. no. 115614[J].Nanotechnology,2007(11):15614-0. |
| [10] | T.Ramanathan;F.T.Fisher;R.S.Ruoff;L.C.Brinson .Amino-Functionalized Carbon Nanotubes for Binding to Polymers and Biological Systems[J].Chemistry of Materials,2005(6):1290-1295. |
| [11] | Sobhi Daniel;Talasila Prasada Rao;Kota Sreenivasa Rao;Sikhakolli Usha Rani;G.R.K. Naidu;Hea-Yeon Lee;Tomoji Kawai .A review of DNA functionalized/grafted carbon nanotubes and their characterization[J].Sensors and Actuators, B. Chemical,2007(2):672-682. |
| [12] | Bianco A;Kostarelos K;Prato M .Applications of carbon nanotubes in drug delivery[J].Current opinion in chemical biology,2005(6):674-679. |
| [13] | Wei Wu;Sebastien Wieckowski;Giorgia Pastorin;Monica Benincasa;Cedric Klumpp .Targeted Delivery of Amphotericin B to Cells by Using Functionalized Carbon Nanotubes[J].Angewandte Chemie,2005(39):6358-6362. |
| [14] | Baker S E;Cai W;Lassreter T L et al.Covalently bonded adducts of deoxyribonucleic acid (DNA) oligonucleotides with single-wall carbon nanotubes:synthesis and hybridization[J].NANO LETTERS,2002,2:1413-1417. |
| [15] | Williams K A;Veenhuizen P T M;de la Torre B et al.Nanotechnology:Carbon nanotubes with DNA recognition[J].NATURE,2002,420:761. |
| [16] | Maruyama H;Yoshimura SH;Akita S;Nagataki A;Nakayama Y .Covalent attachment of protein to the tip of a multiwalled carbon nanotube without sidewall decoration[J].Journal of Applied Physics,2007(9):94701-1-94701-5-0. |
| [17] | Weijie Huang;Shelb Taylor;Kefu Fu;Yi Lin;Donghui Zhang;Timothy W. Hanks;Apparao M. Rao;Ya-Ping Sun .Attaching Proteins to Carbon Nanotubes via Diimide-Activated Amidation[J].Nano letters,2002(4):311-314. |
| [18] | Kuiyang Jiang;Linda S.Schadler;Richard W.Siegel;Xinjie Zhang;Haifeng Zhang;Mauricio Terrones .Protein immobilization on carbon nanotubes via a two-step process of diimide-activated amidation[J].Journal of Materials Chemistry: An Interdisciplinary Journal dealing with Synthesis, Structures, Properties and Applications of Materials, Particulary Those Associated with Advanced Technology,2004(1):37-39. |
| [19] | Mei Li;Erik Dujardin;Stephen Mann .Programmed assembly of multi-layered protein/nanoparticle-carbon nanotube conjugates[J].Chemical communications,2005(39):4952-4954. |
| [20] | Krajcik R;Jung A;Hirsch A;Neuhuber W;Zolk O .Functionalization of carbon nanotubes enables non-covalent binding and intracellular delivery of small interfering RNA for efficient knock-down of genes.[J].Biochemical and Biophysical Research Communications,2008(2):595-602. |
| [21] | Srivastava A;Srivastava O N;Talapatra S et al.Carbon nanotube filter[J].Nature Mat,2004,3:610-613. |
| [22] | Awasthi K;Srivastava A;Srivastava ON .Synthesis of carbon nanotubes[J].Journal of nanoscience and nanotechnology,2005(10):1616-1636. |
| [23] | Luqi Liu;Yujun Qin;Zhi-Xin Guo;Daoben Zhu .Reduction of solubilized multi-walled carbon nanotubes[J].Carbon: An International Journal Sponsored by the American Carbon Society,2003(2):331-335. |
| [24] | Shaffer MSP.;Windle AH.;Fan X. .Dispersion and packing of carbon nanotubes[J].Carbon: An International Journal Sponsored by the American Carbon Society,1998(11):1603-1612. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%