Electricity production from brewery wastewater using dual-chamber microbial fuel cells (MFCs) with a tin-coated copper mesh in the anode was investigated by changing the hydraulic retention time (HRT). The MFCs were fed with wastewater samples from the inlet (inflow, MFC-1) and outlet (outflow, MFC-2) of an anaerobic digester of a brewery wastewater treatment plant. Both chemical oxygen demand removal and current density were improved by decreasing HRT. The best MFC performance was with an HRT of 0.5 d. The maximum power densities of 8.001 and 1.843 μW/cm2 were obtained from reactors MFC-1 and MFC-2, respectively. Microbial diversity at different condi-tions was studied using PCR-DGGE profiling of 16S rRNA fragments of the microorganisms from the biofilm on the anode electrode. The MFC reactor had mainlyGeobacter,Shewanella, andClostridium species, and some bacteria were easily washed out at lower HRTs. The fouling characteristics of the MFC Nafion membrane and the resulting degradation of MFC performance were examined. The ion exchange capacity, conductivity, and diffusivity of the membrane decreased significantly after foul-ing. The morphology of the Nafion membrane and MFC degradation were studied using scanning electron microscopy and attenuated total reflection-Fourier transform infrared spectroscopy.
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