材料期刊网

Carbon/carbon (C/C) composites were deposited with graphite-like carbon (GLC) coating,and then,Arg-Gly-Asp acid (RGD) peptides were successfully immobilized onto the functionalized GLC coating.GLC coating was utilized to prevent carbon particles releasing and create a uniform surface condition for C/C composites.RGD peptides were utilized to improve biocompatibility of GLC coating.Surface chemical characterizations of functionalized GLC coating were detected by contact angle measurement,X-ray photoelectron spectroscopy and Raman spectra.Optical morphology of GLC coatings was observed by confocal laser scanning microscopy.In vitro biological performance was determined using samples seeded with MC3T3-E1 osteoblast-like cells and cultured for 1 week.Surface characterizations and morphological analysis indicated that C/C composites were covered by a dense and uniform GLC coating.Contact angle of GLC coating was reduced to 27.2° when it was functionalized by H2O2 oxidation at 40 ℃ for 1 h.In vitro cytological test showed that the RGD peptides immobilized GLC coating had a significant improvement in biocompatibility.It was suggested that RGD peptides provided GLC coating with a bioactive surface to improve cell adhesion and proliferation on C/C composites.