This work demonstrated quantitatively that DSA (dansylamide) and apo CA (dezincified carbon anhydrase) interacted with each other in the presence of Zn2+ (zinc ion). Thefl uorescence emission of DSA in the presence of Zn2+ and the intensity offl uorescence, which was proportional to the concentration of Zn2+, could be used in the measurement of Zn2+ concentration. Considering that Zn2+ was the active center of enzymes like CA (carbon anhydrase), two dezincifi cation reagents were compared to ensure the validity of our method. A certain range concentration of Zn2+ could be measured by the reaction product of apo CA, DSA and Zn2+, where the measurement limitation was about 60 nmol/L. Interaction of CA with DSA was also studied with infrared spectrometry.
参考文献
| [1] | Hirano T.;Urano Y.;Higuchi T.;Nagano T.;Kikuchi K. .Highly zinc-selective fluorescent sensor molecules suitable for biological applications[J].Journal of the American Chemical Society,2000(49):12399-12400. |
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