A new Quantum Dots(Qdots) nanocrystal composed of semiconductor core and zinc sulfide shell, and its feasibility as labels in immunofluorescence analysis for the imaging of tumor biomarkers by laser scanning confocal microscope(LSCM) was investigated. Qdots taged by mer-captoacetic acid were conjugated with second antibody, then imaging differences of Heat Shock Proteins 70(HSP70) in renal carcinoma tissure sections with immunofluorescence analysis method using Qdots bioconjugates and conventional organic dye FITC were observed by LSCM to assess the brightness and optical stability of Qdots. The experimental results showed Qdots bioconjugates achieved the better results in demonstrating HSP70 with more brighter color and more clear picture than FITC labels. Moreover, the label signals of Qdots did not fade clearly after continued exposure to a 488 nm laser for 1 h. The Qdots bioconjugates have good feasibility in immunofluorescence analysis for the bioimaging by LSCM.
参考文献
| [1] | Ramp U;Mahotka C;Heikaus S;Shibata T;Grimm MO;Willers R;Gabbert HE .Expression of heat shock protein 70 in renal cell carcinoma and its relation to tumor progression and prognosis.[J].Histology and histopathology,2007(10):1099-1107. |
| [2] | 张杰,田波,葛名欢,扬吉伟,张孝斌.不同热休克蛋白在肾细胞癌组织中的表达及其意义[J].中国组织化学与细胞化学杂志,2003(03):272-275. |
| [3] | Pitsillides CM;Joe EK;Wei X;Anderson RR;Lin CP .Selective cell targeting with light-absorbing microparticles and nanoparticles.[J].Biophysical Journal,2003(6):4023-4032. |
| [4] | Nie S .In vivo molecular and cellular imaging with quantum dots.[J].Current Opinion in Biotechnology,2005(1):63-72. |
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