建立了柱前衍生-酸诱导瞬时等速堆积柱上富集毛细管电泳测定巯基化合物的方法。在Tris-HCl缓冲溶液(pH=8.15)中,巯基化合物与2-氯-1-甲基碘化吡啶在室温条件下反应10 min;随后,将反应混合物引入到充满40 mmol/L甲酸盐背景电解质溶液(pH=3.96)的毛细管柱内;最后,向毛细管柱中引入适量稀H2SO4溶液。当在毛细管柱两端施加正向电压时,衍生的巯基化合物就会经瞬时等速堆积电泳过程而发生富集,其中前导和尾随离子分别为H+和Tris+。以半胱氨酸和巯基乙酸为分析对象,检测波长设为312 nm,优化了酸诱导瞬时等速堆积柱上富集的各种实验条件。在最佳条件下,半胱氨酸和巯基乙酸的线性范围分别为10~100 mg/L和4~100 mg/L,检出限分别为0.2和0.4 mg/L。本方法用于脱毛膏中巯基乙酸的测定,结果令人满意。
参考文献：
[1] Go Y M, Jones D P. Free Radical Bio. Med., 2011,50(4):495-509
[2] Millea P J. Am. Fam. Physician, 2009,80(3):265-269
[3] Tang M C, Cheng L, Qiu L, Jia R G, Sun R Q, Wang X P, Hu G Y, Zhao Y. Eur. Rev. Med. Pharmaco. Sci., 2014,18(2):160-164
[4] Tan S T, Itinteang T, Day D J, O’ Donnell C, Mathy J A, Leadbitter P. Brit. J. Dermatol., 2012,167(3):619-624
[5] Besouw M, Masereeuw R, Van den heuvel L, Levtchenko. Drug Discov. Today, 2013,18(15):785-792
[6] Chakor R T, Bharote H, Eklare N, Tamboli K. Ann. Indian Acad. Neur., 2015,18(1):115-116
[7] YANG Xin-Ling, MA Li-Jun, NIU Jia-Rong, WANG Xiao-Yuan. Wool Text. J., 2015,43(5):22-25
杨心玲, 马丽君, 牛家嵘, 王晓渊.毛纺科技,2015,43(5):22-25
[8] XIE Chan, JIA Zhi-Xin, LUO Yuan-Fang, JIA De-Min. Acta Polymerica Sinica, 2011,(3):320-326
谢 婵, 贾志欣, 罗远芳, 贾德民.高分子学报,2011,(3):320-326
[9] Elkhalifa A E O, EI-Tinay A H. Food Chem., 2002,77(2):133-137
[10] Huang K J, Han C H, Han C Q, Li J, Wu Z W, Liu Y M. Microchim Acta, 2011,174(3-4):421-427
[11] Cavrini V, Andrisano V, Gatti R, Scapini G. Int. J. Cosmetic. Sci., 1990,12(4):141-150
[12] Xie N, Ding X, Wang X, Wang P, Zhao S, Wang Z. J. Pharmaceut. Biomed. Anal., 2014,88:509-512
[13] Li M D, Cheng T L, Tseng W L. Electrophoresis, 2009,30(2):388-395
[14] Chang C W, Tseng W L. Anal. Chem., 2010,82(7):2696-2702
[15] Kubalczyk P, Bald E, Furmaniak P, Glowacki R. Anal. Methods, 2014,6(12):4138-4143
[16] Kubalczyk P, Bald E. Electrophoresis, 2009,30(13):2280-2283
[17] Kubalczyk P, Chwatko G, Bald E. Curr. Anal. Chem., 2014,10(3):375-380
[18] Bald E, Kaniowska E, Chwatko G, Glowacki R. Talanta, 2000,50(6):1233-1243
[19] Chwatko G, Bald E. Talanta, 2000,52(3):509-515
[20] Wang X, Chen Y. J. Chromatogr. A, 2009,1216(43):7324-7328
[21] Quirino J P, Breadmore M C. J. Sep. Sci., 2012, 35(1):60-65
[22] Arribas A S, Moreno M, Bermejo E, Zapardiel A, Chicharro M. Electrophoresis, 2011,32(2):275-283
[23] Lu Y, Bai H, Kong C, Zhang H, Breadmore M C. Electrophoresis, 2013,34(24):3326-3332
[24] Hygienic Standard for Cosmetics. Documents of Ministry of Health People’s Republic of China 2007:68-68
化妆品卫生规范.中国人民共和国卫生部. 2007:68-68
[25] Methods for Determination of Thioglycolic Acid in Cosmetics. Documents of China’s Food and Drug Administration. Accessory Four, 2012:17-19
化妆品中巯基乙酸的检测方法.国家食品药品监督管理总局. 附件四,2012:17-19
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