建立了双功能试剂2-[(4-异硫氰基苯基)甲基]-1,4,7,10-四氮杂环十二烷-1,4,7,10-四乙酸(p-SCN-Bn-DOTA)与标准肽段反应的新方法,测定了反应产物与镧系金属Eu(Ⅲ)的螯合效率,探索了其作为靶向探针和蛋白定量标记试剂性能。考察了不同缓冲体系的浓度及pH值、p-SCN-Bn-DOTA量与肽段相对量、反应体系中有机相与水相比例,反应温度与反应时间对偶联效率的影响。实验结果显示:p-SCN-Bn-DOTA量为肽段的32倍,缓冲体系为0.2 mol/L TEAB (pH 8.5),60℃反应60 m{"currentpage":1,"firstResult":0,"maxresult":10,"pagecode":5,"pageindex":{"endPagecode":5,"startPagecode":1},"records":[{"abstractinfo":"介绍了广泛应用于电子行业的电子浆料,着重介绍了电子浆料中可以替代贵金属导电相的铜(Cu)、镍(Ni)和铝(Al)等贱金属导电相.Cu、Ni和Al贱金属在空气中易氧化,从而导致其电导率下降,限制了其在电子浆料中的应用.以不同保护机理为基础,总结了目前可以改善Cu、Ni和Al贱金属电导率的工艺,提出环保型、高性能、低成本的电子浆料是今后的发展趋势.","authors":[{"authorName":"李冰","id":"a4bb195c-a3ac-43b6-875f-a9160b9d529e","originalAuthorName":"李冰"},{"authorName":"王俊勃","id":"085d9512-5d7f-4b08-b5f4-9baf59f62f4c","originalAuthorName":"王俊勃"},{"authorName":"苏晓磊","id":"a1dfd1b0-656a-4372-8c0f-bfb5d481603a","originalAuthorName":"苏晓磊"},{"authorName":"高照元","id":"f289f229-2162-482d-b1d4-72d722a71110","originalAuthorName":"高照元"},{"authorName":"王蒙","id":"66c8b395-30ff-4281-838a-6121cfe76d77","originalAuthorName":"王蒙"},{"authorName":"王伦","id":"9af9f1d5-c5de-4890-b0ad-2ad904e38d8d","originalAuthorName":"王伦"}],"doi":"","fpage":"35","id":"1670bf74-981a-494b-ab46-9e5ddd17aea8","issue":"21","journal":{"abbrevTitle":"CLDB","coverImgSrc":"journal/img/cover/CLDB.jpg","id":"8","issnPpub":"1005-023X","publisherId":"CLDB","title":"材料导报"},"keywords":[{"id":"90476ad3-7e0a-4ac4-8ce0-fa38a2ee0f17","keyword":"电子浆料","originalKeyword":"电子浆料"},{"id":"6f377414-5e20-410d-8b04-78a259034b21","keyword":"导电相","originalKeyword":"导电相"},{"id":"e5ff4cb0-aacd-4857-9a61-8b06320256c4","keyword":"工艺","originalKeyword":"工艺"}],"language":"zh","publisherId":"cldb201321007","title":"电子浆料中的贱金属导电相的改善工艺研究进展","volume":"27","year":"2013"},{"abstractinfo":"利用透射电镜和X射线衍射技术研究了一种低碳微合金钢中Nb、Ti碳氮化物的回溶行为.研究结果表明,随着加热温度的升高,保温时间的延长,第二相粒子发生回溶,第二相粒子的数量减少,尺寸也逐渐变细小.同时,粒子形貌由主要呈近球形逐渐变为主要呈方形.加热过程中,Nb和Ti的碳氮化物均会发生溶解,Nb的回溶速度要比Ti快.","authors":[{"authorName":"吴新朗","id":"332a3c1c-15dd-43c4-9ffc-9a53192adf6e","originalAuthorName":"吴新朗"},{"authorName":"赵征志","id":"c67581a7-0ff4-49dd-88b1-8d0dcba604dd","originalAuthorName":"赵征志"},{"authorName":"余威","id":"d75a42a1-022b-4e53-b41c-5aa2e8b4e4a1","originalAuthorName":"余威"},{"authorName":"李彬","id":"a689218b-7c89-46a9-b453-d8f8b0df80be","originalAuthorName":"李彬"},{"authorName":"王伦","id":"667b2fba-656e-4d60-8c26-d0c6a0b6ce34","originalAuthorName":"王伦"}],"doi":"","fpage":"29","id":"60667890-640a-4868-a9de-b496ae8bee13","issue":"1","journal":{"abbrevTitle":"GTFT","coverImgSrc":"journal/img/cover/gtft1.jpg","id":"28","issnPpub":"1004-7638","publisherId":"GTFT","title":"钢铁钒钛"},"keywords":[{"id":"2c5a83be-1dc2-4574-81e7-6cd75c7497ac","keyword":"微合金钢","originalKeyword":"微合金钢"},{"id":"47e20bb7-0c8c-49ee-8293-c7cdfc2a6b3f","keyword":"第二相粒子","originalKeyword":"第二相粒子"},{"id":"acd3c230-9c47-4883-b542-549c1ff4159b","keyword":"加热工艺","originalKeyword":"加热工艺"},{"id":"86847b5c-5dff-40d5-8604-01b0df427acb","keyword":"回溶行为","originalKeyword":"回溶行为"},{"id":"5761ff53-c39a-4600-a703-489d0431231b","keyword":"碳氮化物","originalKeyword":"碳氮化物"}],"language":"zh","publisherId":"gtft200901006","title":"低碳微合金钢中Nb、Ti碳氮化物的回溶行为研究","volume":"30","year":"2009"},{"abstractinfo":"提出合成新型荧光试剂N-9-吖啶基氨基乙酸的3种不同方法,其中用9-苯氧基叮啶和氨基乙酸钠作用的间接合成法产率达78.9%. 目标产物通过红外、核磁共振等进行了结构表征,并对其荧光性质和紫外光谱进行了初步探讨. 结果表明,浓度为5.0×10-7 mol/L的N-9-吖啶基氨基乙酸标准溶液在pH=9.0的NH4Cl/NH3*H2O体系中荧光强度(F)最大,F值可达1 500以上,λex和λem分别为268和460 nm;在209、253和385 nm处有明显的紫外-可见吸收峰.","authors":[{"authorName":"周运友","id":"b916a1be-c40e-4786-a3f4-d7e88110104c","originalAuthorName":"周运友"},{"authorName":"严正权","id":"1a2c2e5a-90cf-47af-b8a6-f3579f720c82","originalAuthorName":"严正权"},{"authorName":"汪乐余","id":"d527ba70-0c1f-47c7-8604-85b67982b77a","originalAuthorName":"汪乐余"},{"authorName":"王伦","id":"069c5dc2-faba-4e77-bec0-7729f71b27ea","originalAuthorName":"王伦"},{"authorName":"佘世科","id":"9c294ea3-9a50-4765-9bbe-b8ff718feb3f","originalAuthorName":"佘世科"},{"authorName":"胡蕾","id":"d95ac468-845b-44b7-a032-1a8e93217d5f","originalAuthorName":"胡蕾"}],"doi":"10.3969/j.issn.1000-0518.2003.12.012","fpage":"1176","id":"3c14b465-8661-4e91-9f08-896f75303ff2","issue":"12","journal":{"abbrevTitle":"YYHX","coverImgSrc":"journal/img/cover/YYHX.jpg","id":"73","issnPpub":"1000-0518","publisherId":"YYHX","title":"应用化学"},"keywords":[{"id":"80b8a342-90cf-4f40-9a13-990d0613f88f","keyword":"N-吖啶基氨基乙酸","originalKeyword":"N-吖啶基氨基乙酸"},{"id":"ad3e89f3-8ded-49f2-ab25-a7ed83dfdbbf","keyword":"合成","originalKeyword":"合成"},{"id":"6e5d6575-13f1-4a0e-ab4d-874833f21374","keyword":"荧光试剂","originalKeyword":"荧光试剂"},{"id":"611b21f7-0663-4a9a-a226-5939b8fdddae","keyword":"光谱","originalKeyword":"光谱"}],"language":"zh","publisherId":"yyhx200312012","title":"新型荧光试剂N-9-吖啶基氨基乙酸的合成及其光谱性质","volume":"20","year":"2003"},{"abstractinfo":"","authors":[{"authorName":"陈红旗","id":"49be19f4-1229-47ab-9c26-033d955ec090","originalAuthorName":"陈红旗"},{"authorName":"汪乐余","id":"3e48fb0b-e036-472b-a488-9a5f558f00fa","originalAuthorName":"汪乐余"},{"authorName":"李玲","id":"4c08ed9d-ca80-4f01-949a-fd30bf669f4b","originalAuthorName":"李玲"},{"authorName":"夏婷婷","id":"6dee10c9-3219-4fb6-9917-4e6ce2622b0f","originalAuthorName":"夏婷婷"},{"authorName":"王伦","id":"1e6baf84-75ae-4193-b472-ce2546744de7","originalAuthorName":"王伦"}],"doi":"10.3969/j.issn.1000-0518.2004.02.020","fpage":"197","id":"d706e6f8-7b8a-40b1-a865-be19f7c16b8f","issue":"2","journal":{"abbrevTitle":"YYHX","coverImgSrc":"journal/img/cover/YYHX.jpg","id":"73","issnPpub":"1000-0518","publisherId":"YYHX","title":"应用化学"},"keywords":[{"id":"112d6e21-302f-4930-b4a2-151c3ed23e57","keyword":"苯乙烯","originalKeyword":"苯乙烯"},{"id":"501ae6a5-396f-408e-8770-4cf3ef581007","keyword":"丙烯酸","originalKeyword":"丙烯酸"},{"id":"17771251-96b8-40b0-bf38-b8128ba6deb5","keyword":"元皂乳液聚合","originalKeyword":"元皂乳液聚合"},{"id":"7de63e01-fbd8-4bae-819d-7160882a7c40","keyword":"超声辐射","originalKeyword":"超声辐射"},{"id":"740a70be-3e09-4435-b4be-64aadc844a51","keyword":"纳米粒子","originalKeyword":"纳米粒子"},{"id":"e9ab7c00-75b8-45ff-9401-ac8152a3dd62","keyword":"共振光散射","originalKeyword":"共振光散射"}],"language":"zh","publisherId":"yyhx200402020","title":"聚苯乙烯-丙烯酸纳米粒子的制备及共振光散射性质","volume":"21","year":"2004"},{"abstractinfo":"在超声辐照下,用过氧化氢对聚乙烯醇(PVA)氧化降解,制得纳米尺寸的β-二酮型高分子微球(PVK),红外光谱表征其β-二酮型结构为烯醇式,透射电镜观察到该纳米粒子平均直径为20 nm. 对PVK的共振散射光性质研究发现,pH=3.0时,PVK与蛋白质形成缔合微粒时将导致PVK 380 nm处RLS信号急剧增加,其强度增加值与蛋白质的浓度呈线性关系,据此可以建立一种测定蛋白质的新方法. 测得HSA和γ-G的线性范围分别为0～15.0 ×10-6 g/mL和0.2×10-6～8.5×10-6 g/mL,检出限为1.4×10-9 g/mL和17.5×10-9 g/mL. 本法应用于人血清样品中蛋白质的定量分析,结果满意.","authors":[{"authorName":"周运友","id":"38abae0c-f8f2-4bb5-a4c7-e569586d54b6","originalAuthorName":"周运友"},{"authorName":"佘世科","id":"dde42635-4729-4765-a229-1ac4839b6ff1","originalAuthorName":"佘世科"},{"authorName":"章丽","id":"e9f414c6-2e6a-4c20-b2e5-0597c0784fba","originalAuthorName":"章丽"},{"authorName":"严正权","id":"2c45ce6b-e968-4b4f-ba4b-5289a939e0d8","originalAuthorName":"严正权"},{"authorName":"卢琴","id":"6f8f5467-1d49-4014-a949-e510a91c4ab6","originalAuthorName":"卢琴"},{"authorName":"王伦","id":"c0f0f5a8-18a7-412e-9c4d-e13c4972a777","originalAuthorName":"王伦"}],"doi":"10.3969/j.issn.1000-0518.2004.12.005","fpage":"1225","id":"736e1720-66b6-48e2-a182-d92c06cc7163","issue":"12","journal":{"abbrevTitle":"YYHX","coverImgSrc":"journal/img/cover/YYHX.jpg","id":"73","issnPpub":"1000-0518","publisherId":"YYHX","title":"应用化学"},"keywords":[{"id":"f62bb97f-7fdd-46ef-9f48-57a31458eda9","keyword":"高分子纳米粒子","originalKeyword":"高分子纳米粒子"},{"id":"fc3a9a02-2ef8-4741-b37e-972c7ca3df7e","keyword":"聚乙烯醇","originalKeyword":"聚乙烯醇"},{"id":"e0ad1647-0377-45cd-aaa4-4f4c8d993fba","keyword":"β-二酮","originalKeyword":"β-二酮"},{"id":"1d1fafde-1a22-435d-af1f-7f31b158f89d","keyword":"蛋白质","originalKeyword":"蛋白质"},{"id":"0c1be3d6-395d-4ad4-944e-eba1fb612acb","keyword":"共振散射光","originalKeyword":"共振散射光"}],"language":"zh","publisherId":"yyhx200412005","title":"功能性β-二酮型高分子纳米微球的制备及其共振散射光性质","volume":"21","year":"2004"},{"abstractinfo":"由苯并三唑、乙二醛、氨基脲合成了新荧光试剂二(苯并三唑-1-基)乙二酮缩氨基脲希夫碱. 通过1H NMR、红外光谱、元素分析确证了试剂的结构. 在pH=4.0的KH2PO4-H3PO4缓冲溶液中,该试剂在342 nm处(λex=284 nm)发射荧光,其强度随蛋白质的加入明显增强,据此建立了测定痕量蛋白质的新方法. 在最佳实验条件下,测定牛血清白蛋白、人血清白蛋白的线性范围分别为0～8.0×10-6 g/mL和1.0×10-5～0.25 g/mL ,检测限分别为5.3×10-9 g/mL、1.4×10-9 g/mL,用于合成样品的测定,结果令人满意.","authors":[{"authorName":"高峰","id":"8324c9d5-ee3e-4b2c-bec8-d2e821a40830","originalAuthorName":"高峰"},{"authorName":"朱英贵","id":"0a82d9c3-cac1-4cea-9fdb-f2be9890ec40","originalAuthorName":"朱英贵"},{"authorName":"章丽","id":"b85a80f9-b782-4283-858d-56d93d5cdfca","originalAuthorName":"章丽"},{"authorName":"谢筱娟","id":"eaf52460-cc1f-4e10-870a-c485f07cd3bb","originalAuthorName":"谢筱娟"},{"authorName":"王伦","id":"dcb21a67-aae3-4d8a-a6a2-22f603e9d4d4","originalAuthorName":"王伦"}],"doi":"10.3969/j.issn.1000-0518.2004.04.011","fpage":"374","id":"d5f81392-e61f-4b89-aad7-c9e09ddd2c9c","issue":"4","journal":{"abbrevTitle":"YYHX","coverImgSrc":"journal/img/cover/YYHX.jpg","id":"73","issnPpub":"1000-0518","publisherId":"YYHX","title":"应用化学"},"keywords":[{"id":"722bf5f3-430b-41bf-9a7c-ca119f3a02a4","keyword":"二(苯并三唑基)乙二酮缩氨基脲","originalKeyword":"二(苯并三唑基)乙二酮缩氨基脲"},{"id":"5e6117ec-b7fb-4da2-b5b5-1119f1afcde5","keyword":"蛋白质","originalKeyword":"蛋白质"},{"id":"a4d226d3-3a34-470b-a102-f407178ba099","keyword":"荧光光度法","originalKeyword":"荧光光度法"}],"language":"zh","publisherId":"yyhx200404011","title":"荧光试剂二(苯并三唑-1-基)乙二酮缩氨基脲的合成及其在蛋白质测定中的应用","volume":"21","year":"2004"},{"abstractinfo":"对阳离子荧光染料酚藏花红(PF)在阴离子表面活性剂溶液状态的吸收光谱和荧光光谱进行了研究.结果表明,当阴离子表面活性剂十二烷基硫酸钠(SDS)的浓度在0.8×10-3～2.0×10-3 mol/L范围内时,酚藏花红形成非荧光现场二聚体,且在515 nm处出现新的特征吸收峰.现场形成的酚藏花红二聚体作为荧光探针的实验表明,核酸可使体系的荧光强度有很大的增强,且其增强的程度与核酸加入量呈明显的线性关系,据此建立了核酸定量分析的新方法.在最优化条件下,用于小牛胸腺DNA、鲱鱼精DNA、酵母RNA的定量测定,其线性范围分别为0.06～18 mg/L、0.04～20 mg/L和0.12～20 mg/L;检测限分别为21、17和36μg/L.","authors":[{"authorName":"高峰","id":"fc6c9ae1-f6f1-43c3-b87e-43d82f9c2c99","originalAuthorName":"高峰"},{"authorName":"杜俊","id":"bc500efb-a689-495a-8555-90ecc3ee13ff","originalAuthorName":"杜俊"},{"authorName":"章丽","id":"a1441f4b-6617-40d2-9750-6d9658067985","originalAuthorName":"章丽"},{"authorName":"卞桂荣","id":"f22199a4-6cb1-46f8-b27c-5e8795390225","originalAuthorName":"卞桂荣"},{"authorName":"朱昌青","id":"f9a0d64b-bd5a-4115-8dd8-f556748fc676","originalAuthorName":"朱昌青"},{"authorName":"王伦","id":"1b5bada1-c971-4557-be84-4bca623c50ce","originalAuthorName":"王伦"}],"doi":"10.3969/j.issn.1000-0518.2004.07.016","fpage":"722","id":"daa4dbb0-0b19-4caa-91bd-409efd6021bf","issue":"7","journal":{"abbrevTitle":"YYHX","coverImgSrc":"journal/img/cover/YYHX.jpg","id":"73","issnPpub":"1000-0518","publisherId":"YYHX","title":"应用化学"},"keywords":[{"id":"e794f29e-423b-4d9e-a84c-596c6a63946b","keyword":"酚藏花红","originalKeyword":"酚藏花红"},{"id":"cb50945a-0b02-4398-a291-c83ee7bb5bab","keyword":"阴离子表面活性剂","originalKeyword":"阴离子表面活性剂"},{"id":"cfc51cf5-c0ee-4499-8ebe-8c8f1986390e","keyword":"核酸","originalKeyword":"核酸"},{"id":"3ba5e716-50f6-4ffc-ad60-2b8cf2e0bc7e","keyword":"荧光分析法","originalKeyword":"荧光分析法"}],"language":"zh","publisherId":"yyhx200407016","title":"阴离子表面活性剂与酚藏花红的作用及其在核酸荧光法测定中的应用","volume":"21","year":"2004"},{"abstractinfo":"稀硫酸介质中,荧光染料亮绿SF在312nm处产生荧光峰,Fenton反应产生的·OH能与亮绿SF反应,使其荧光猝灭.在H2SO4溶液pH值为3.5、亮绿SF、Fe2+的浓度均为8.0×10-5mol/L的最佳实验条件下,测定312nm处荧光强度的变化值△F,可间接测定Fenton反应中羟自由基的生成量.结果表明,该法可作为一种简便的筛选羟自由基清除剂的方法,适用于羟自由基的在线测定和监测.","authors":[{"authorName":"高峰","id":"45413bb1-0ae5-4834-8651-299fca9a1eaa","originalAuthorName":"高峰"},{"authorName":"唐莉娟","id":"52802bb9-6f9e-45a1-9ed6-3f24ceb4e3b2","originalAuthorName":"唐莉娟"},{"authorName":"王伦","id":"d1014bc0-5310-41ac-9cc2-26c9cd323ad6","originalAuthorName":"王伦"},{"authorName":"朱昌青","id":"f97a489b-20a3-4787-ac74-1fdf05d05c58","originalAuthorName":"朱昌青"}],"doi":"10.3969/j.issn.1000-0518.2006.04.027","fpage":"453","id":"497479e8-c722-4542-8ff0-0a1ee01da0e7","issue":"4","journal":{"abbrevTitle":"YYHX","coverImgSrc":"journal/img/cover/YYHX.jpg","id":"73","issnPpub":"1000-0518","publisherId":"YYHX","title":"应用化学"},"keywords":[{"id":"94340621-cee1-4075-b437-574017208620","keyword":"芬顿反应","originalKeyword":"芬顿反应"},{"id":"ecdc3749-4a20-4fc5-88ef-23d5df23ff96","keyword":"羟自由基","originalKeyword":"羟自由基"},{"id":"be66b36d-d6bf-4b0b-a5c5-0cbad83c8779","keyword":"亮绿SF","originalKeyword":"亮绿SF"},{"id":"a47f303b-f19a-43a9-8107-3784e7f52077","keyword":"荧光光度法","originalKeyword":"荧光光度法"}],"language":"zh","publisherId":"yyhx200604027","title":"简便荧光法测定Fenton反应产生的羟自由基","volume":"23","year":"2006"},{"abstractinfo":"在水溶液中合成了CdS纳米粒子,利用超声辐射下原位聚合方法合成了聚4-戊烯酸(PPA)包裹的CdS/PPA复合纳米溶胶.作为荧光探针,建立了同步荧光光度法测定牛血清蛋白的工作曲线ΔF=117.50+29.55c(1×10-6 g/mL),线性范围为0.50×10-6～30×10-6 g/mL,检出限为0.12×10-6 g/mL.对合成样品进行了测定,结果令人满意.","authors":[{"authorName":"陈红旗","id":"beeccd9b-a87a-43a1-b06d-9e66c0fb13f1","originalAuthorName":"陈红旗"},{"authorName":"梁阿妮","id":"fb1ff681-b622-4873-8b9d-be391c9a0923","originalAuthorName":"梁阿妮"},{"authorName":"许轶","id":"fdc5bda7-fc55-4d13-9350-8f3fc8aa553d","originalAuthorName":"许轶"},{"authorName":"王伦","id":"7f52d1cc-cbf3-462e-8483-8dd1cfd8cd87","originalAuthorName":"王伦"}],"doi":"10.3969/j.issn.1000-0518.2008.12.023","fpage":"1484","id":"27c77c1a-3119-4744-980c-8b126f510961","issue":"12","journal":{"abbrevTitle":"YYHX","coverImgSrc":"journal/img/cover/YYHX.jpg","id":"73","issnPpub":"1000-0518","publisherId":"YYHX","title":"应用化学"},"keywords":[{"id":"7399dcb1-8f46-4ca2-80a2-3a5047e79400","keyword":"同步荧光光谱","originalKeyword":"同步荧光光谱"},{"id":"6d059b65-34ad-43ac-b8a1-7008cffafd87","keyword":"CdS/PPA纳米粒子","originalKeyword":"CdS/PPA纳米粒子"},{"id":"308339d9-f5ce-4c8e-9e0b-ce4e3ceacfa4","keyword":"牛血清白蛋白","originalKeyword":"牛血清白蛋白"}],"language":"zh","publisherId":"yyhx200812023","title":"CdS/PPA纳米溶胶荧光探针同步荧光光度法测定水溶液中牛血清白蛋白","volume":"25","year":"2008"},{"abstractinfo":"海南抱伦炭浆厂,建成投产后,通过采取正确的生产调试措施,经一个月的生产调试,使生产就达到了设计指标:处理能力230t/d,金总回收率93.04%.","authors":[{"authorName":"赵国铭","id":"02d93390-5bfd-43bf-b93c-a4a2ba384e24","originalAuthorName":"赵国铭"}],"doi":"10.3969/j.issn.1001-1277.2004.10.014","fpage":"43","id":"349ec3f8-223f-4588-83a8-e44cfd1ae53e","issue":"10","journal":{"abbrevTitle":"HJ","coverImgSrc":"journal/img/cover/HJ.jpg","id":"44","issnPpub":"1001-1277","publisherId":"HJ","title":"黄金"},"keywords":[{"id":"8f57d6af-bf6f-411c-b56e-c86b3345cf63","keyword":"炭浆工艺","originalKeyword":"炭浆工艺"},{"id":"27fbce1e-884d-4496-a371-a3d418eacfc0","keyword":"生产调试","originalKeyword":"生产调试"},{"id":"3aaec0dd-9e83-4068-aa6c-31072f688898","keyword":"生产指标","originalKeyword":"生产指标"}],"language":"zh","publisherId":"huangj200410014","title":"抱伦炭浆厂生产调试实践","volume":"25","year":"2004"}],"totalpage":12,"totalrecord":117}